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Applied and Environmental Microbiology, October 2003, p. 6299-6301, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.6299-6301.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Development of a mariner-Based Transposon for Use in Sorangium cellulosum

Bryan Julien* and Ruby Fehd

Kosan Biosciences, Inc., Hayward, California 94545

Received 12 May 2003/ Accepted 28 July 2003

In order to generate marked insertions in the myxobacterium Sorangium cellulosum, a transposon based on the eukaryotic mariner transposon was developed. The transposition frequency was increased with the use of a mutated tnp gene. The transposon randomly inserts into the chromosome, as demonstrated by targeted mutagenesis of the epoK gene.

* Corresponding author. Mailing address: Kosan Biosciences, Inc., 3832 Bay Center Pl., Hayward, CA 94545. Phone: (510) 731-5209. Fax: (510) 732-8401. E-mail: julien{at}kosan.com.

Applied and Environmental Microbiology, October 2003, p. 6299-6301, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.6299-6301.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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