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Applied and Environmental Microbiology, October 2003, p. 6337-6339, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.6337-6339.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Petroleum Pollution Bioremediation Using Water-Insoluble Uric Acid as the Nitrogen Source

Omry Koren, Vishnia Knezevic, Eliora Z. Ron, and Eugene Rosenberg*

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel 69978

Received 30 April 2003/ Accepted 9 July 2003

The biodegradation of hydrocarbon pollutants in open systems is limited by the availability of a utilizable nitrogen source. This limitation can be overcome by using uric acid. Enrichment cultures grown on crude oil-uric acid media yielded mixed and pure cultures that degraded petroleum. In a simulated open system, uric acid bound to crude oil and was available for bacterial growth and petroleum biodegradation.


* Corresponding author. Mailing address: Department of Molecular Microbiology & Biotechnology, Tel Aviv University, Ramat Aviv 69978, Israel. Phone: 972-3-640 9838. Fax: 972-3-642 9377. E-mail: eros{at}post.tau.ac.il.


Applied and Environmental Microbiology, October 2003, p. 6337-6339, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.6337-6339.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.