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Applied and Environmental Microbiology, November 2003, p. 6370-6379, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6370-6379.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing for Comparison of Veterinary and Human Isolates of Campylobacter jejuni

Georgina Manning,1 Christopher G. Dowson,2 Mary C. Bagnall,1 If H. Ahmed,1 Malcolm West,3 and Diane G. Newell1*

Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB,1 Department of Biological Sciences, University of Warwick, Coventry CV4 7AL,2 Queen's Hospital, Burton-on-Trent, Staffordshire, United Kingdom3

Received 22 January 2003/ Accepted 27 August 2003

Multilocus sequence typing (MLST) has been applied to 266 Campylobacter jejuni isolates, mainly from veterinary sources, including cattle, sheep, poultry, pigs, pets, and the environment, as well as isolates from human cases of campylobacteriosis. The populations of veterinary and human isolates overlap, suggesting that most veterinary sources should be considered reservoirs of pathogenic campylobacters. There were some associations between source and sequence type complex, indicating that host or source adaptation may exist. The pig isolates formed a distinct group by MLST and may well represent a potential pig-adapted clone of C. jejuni. A subset (n = 82) of isolates was reanalyzed with a second MLST scheme which provided a unique set of isolates that had been analyzed at a total of 12 loci. The distribution of isolates among the complexes in each of the two schemes was similar but not identical. In addition to isolates from human outbreaks, one group of isolates that were not epidemiologically linked was also identical at all 12 loci. This group of isolates is believed to represent another stable strain of C. jejuni.

* Corresponding author. Mailing address: Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. Phone: 44 (0)1932 357 547. Fax: 44 (0)1932 357 268. E-mail: d.newell{at}vla.defra.gsi.gov.uk.

Applied and Environmental Microbiology, November 2003, p. 6370-6379, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6370-6379.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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