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Applied and Environmental Microbiology, November 2003, p. 6481-6488, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6481-6488.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Fingerprinting Microbial Assemblages from the Oxic/Anoxic Chemocline of the Black Sea

Costantino Vetriani,1,2 Hiep V. Tran,2 and Lee J. Kerkhof2*

Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, New Jersey 08901-8525,1 Institute of Marine and Coastal Sciences, Rutgers University, New Brunswick, New Jersey 08901-85212

Received 31 March 2003/ Accepted 11 August 2003

Biomass samples from the Black Sea collected in 1988 were analyzed for SSU genes from Bacteria and Archaea after 10 years of storage at -80°C. Both clonal libraries and direct fingerprinting by terminal restriction fragment length polymorphism (T-RFLP) analyses were used to assess the microbial community. Uniform and discrete depth distributions of different SSU phylotypes were observed. However, most recombinant clones were not restricted to a specific depth in the water column, and many of the major T-RFLP peaks remain uncharacterized. Of the clones obtained, an {varepsilon}-Proteobacteria and a Pseudoalteromonas-like clone accounted for major peaks in the fingerprint, while deeply branching lineages of {alpha}- and {gamma}-Proteobacteria were associated with smaller peaks. Additionally, members were found among both the {delta}-Proteobacteria related to sulfate reducers and the Archaea related to phylotypes from the ANME groups that anaerobically oxidize methane.

* Corresponding author. Mailing address: Institute of Marine and Coastal Sciences, Rutgers University, 71 Dudley Rd., New Brunswick, NJ 08901-8521. Phone: (732) 932-6555, ext. 335. Fax: (732) 932-6520. E-mail: kerkhof{at}imcs.rutgers.edu.

Applied and Environmental Microbiology, November 2003, p. 6481-6488, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6481-6488.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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