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Applied and Environmental Microbiology, November 2003, p. 6495-6499, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6495-6499.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Modification of the Monomer Composition of Polyhydroxyalkanoate Synthesized in Saccharomyces cerevisiae Expressing Variants of the ß-Oxidation-Associated Multifunctional Enzyme

Silvia Marchesini,1 Nadine Erard,1 Tuomo Glumoff,2 J. Kalervo Hiltunen,2 and Yves Poirier1*

Laboratoire de Biotechnologie Végétale, Département de Biologie Moléculaire Végétale, Université de Lausanne, CH-1015 Lausanne, Switzerland,1 Biocenter Oulu and Department of Biochemistry, University of Oulu, FIN-90570 Oulu, Finland2

Received 28 April 2003/ Accepted 1 August 2003

Expression by Saccharomyces cerevisiae of a polyhydroxyalkanoate (PHA) synthase modified at the carboxy end by the addition of a peroxisome targeting signal derived from the last 34 amino acids of the Brassica napus isocitrate lyase (ICL) and containing the terminal tripeptide Ser-Arg-Met resulted in the synthesis of PHA. The ability of the terminal peptide Ser-Arg-Met and of the 34-amino-acid peptide from the B. napus ICL to target foreign proteins to the peroxisome of S. cerevisiae was demonstrated with green fluorescent protein fusions. PHA synthesis was found to be dependent on the presence of both the enzymes generating the ß-oxidation intermediate 3-hydroxyacyl-coenzyme A (3-hydroxyacyl-[CoA]) and the peroxin-encoding PEX5 gene, demonstrating the requirement for a functional peroxisome and a ß-oxidation cycle for PHA synthesis. Using a variant of the S. cerevisiae ß-oxidation multifunctional enzyme with a mutation inactivating the B domain of the R-3-hydroxyacyl-CoA dehydrogenase, it was possible to modify the PHA monomer composition through an increase in the proportion of the short-chain monomers of five and six carbons.

* Corresponding author. Mailing address: Laboratoire de Biotechnologie Végétale, Département de Biologie Moléculaire Végétale, Université de Lausanne, CH-1015 Lausanne, Switzerland. Phone: 41 21 692 4222. Fax: 41 21 692 4195. E-mail: yves.poirier{at}ie-bpv.unil.ch.

Applied and Environmental Microbiology, November 2003, p. 6495-6499, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6495-6499.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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