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Applied and Environmental Microbiology, November 2003, p. 6500-6506, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6500-6506.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Reactive Oxygen Species and Induction of Lignin Peroxidase in Phanerochaete chrysosporium

Paula A. Belinky,1 Nufar Flikshtein,1,2 Sergey Lechenko,1 Shimon Gepstein,2 and Carlos G. Dosoretz3*

MIGAL-Galilee Technology Center, Kiryat Shmona 10200,1 Faculty of Biology,2 Division of Environmental Engineering, Infrastructure and Environmental Sciences, Faculty of Civil and Environmental Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel3

Received 7 April 2003/ Accepted 7 August 2003

We studied oxidative stress in lignin peroxidase (LIP)-producing cultures (cultures flushed with pure O2) of Phanerochaete chrysosporium by comparing levels of reactive oxygen species (ROS), cumulative oxidative damage, and antioxidant enzymes with those found in non-LIP-producing cultures (cultures grown with free exchange of atmospheric air [control cultures]). A significant increase in the intracellular peroxide concentration and the degree of oxidative damage to macromolecules, e.g., DNA, lipids, and proteins, was observed when the fungus was exposed to pure O2 gas. The specific activities of manganese superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase and the consumption of glutathione were all higher in cultures exposed to pure O2 (oxygenated cultures) than in cultures grown with atmospheric air. Significantly higher gene expression of the LIP-H2 isozyme occurred in the oxygenated cultures. A hydroxyl radical scavenger, dimethyl sulfoxide (50 mM), added to the culture every 12 h, completely abolished LIP expression at the mRNA and protein levels. This effect was confirmed by in situ generation of hydroxyl radicals via the Fenton reaction, which significantly enhanced LIP expression. The level of intracellular cyclic AMP (cAMP) was correlated with the starvation conditions regardless of the oxygenation regimen applied, and similar cAMP levels were obtained at high O2 concentrations and in cultures grown with atmospheric air. These results suggest that even though cAMP is a prerequisite for LIP expression, high levels of ROS, preferentially hydroxyl radicals, are required to trigger LIP synthesis. Thus, the induction of LIP expression by O2 is at least partially mediated by the intracellular ROS.

* Corresponding author. Mailing address: Division of Environmental Engineering, Infrastructure and Environmental Sciences, Faculty of Civil and Environmental Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel. Phone: 972-4-8294962. Fax: 972-4-8228898. E-mail: carlosd{at}tx.technion.ac.il.

Applied and Environmental Microbiology, November 2003, p. 6500-6506, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6500-6506.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shary, S., Kapich, A. N., Panisko, E. A., Magnuson, J. K., Cullen, D., Hammel, K. E. (2008). Differential Expression in Phanerochaete chrysosporium of Membrane-Associated Proteins Relevant to Lignin Degradation. Appl. Environ. Microbiol. 74: 7252-7257 [Abstract] [Full Text]  
  • Matityahu, A., Hadar, Y., Dosoretz, C. G., Belinky, P. A. (2008). Gene Silencing by RNA Interference in the White Rot Fungus Phanerochaete chrysosporium. Appl. Environ. Microbiol. 74: 5359-5365 [Abstract] [Full Text]  


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