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Applied and Environmental Microbiology, November 2003, p. 6949-6953, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6949-6953.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Agrobacterium Bioassay Strain for Ultrasensitive Detection of N-Acylhomoserine Lactone-Type Quorum-Sensing Molecules: Detection of Autoinducers in Mesorhizobium huakuii

Jun Zhu,1,2* Yunrong Chai,1 Zengtao Zhong,2 Shunpeng Li,2 and Stephen C. Winans1

Department of Microbiology, Cornell University, Ithaca, New York 14853,1 Department of Microbiology, MOA Key Lab of Microbiological Engineering of Agricultural Environment, Nanjing Agricultural University, Nanjing, People's Republic of China2

Received 4 June 2003/ Accepted 4 September 2003

An ultrasensitive bioassay system for the detection of N-acylhomoserine lactones (AHLs) was constructed in Agrobacterium tumefaciens by using the T7 expression system to overproduce the AHL receptor TraR. This strain detected many diverse AHLs, some at extremely low concentrations. We used this strain to detect for the first time AHLs made by Mesorhizobium huakuii, which symbiotically fixes nitrogen in association with the legume Astragalus sinicus, a source of green manure throughout eastern Asia.

* Corresponding author. Mailing address: Department of Microbiology, MOA Key Lab of Microbiological Engineering of Agricultural Environment, Nanjing Agricultural University, Nanjing, People's Republic of China. Phone: 1186-25-4396645. Fax: 1186-25-4396645. E-mail: jun_zhu{at}njau.edu.cn.

Applied and Environmental Microbiology, November 2003, p. 6949-6953, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6949-6953.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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