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Applied and Environmental Microbiology, December 2003, p. 7002-7008, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7002-7008.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification and Distribution of Insertion Sequences of Paracoccus solventivorans

Dariusz Bartosik,* Michal Szymanik, and Jadwiga Baj

Department of Bacterial Genetics, Institute of Microbiology, Warsaw University, 02-096 Warsaw, Poland

Received 15 July 2003/ Accepted 18 September 2003

Three novel insertion sequences (ISs) (ISPso1, ISPso2, and ISPso3) of the soil bacterium Paracoccus solventivorans DSM 11592 were identified by transposition into entrapment vector pMEC1. ISPso1 (1,400 bp) carries one large open reading frame (ORF) encoding a putative basic protein (with a DDE motif conserved among transposases [Tnps] of elements belonging to the IS256 family) with the highest levels of similarity with the hypothetical Tnps of Rhodospirillum rubrum and Sphingopyxis macrogoltabida. ISPso2 (832 bp) appeared to be closely related to ISPpa2 of Paracoccus pantotrophus DSM 11072 and IS1248 of Paracoccus denitrificans PdX22, both of which belong to the IS427 group (IS5 family). These elements contain two overlapping ORFs and a putative frameshift motif (AAAAG) responsible for production of a putative transframe Tnp. ISPso3 (1,286 bp) contains a single ORF, whose putative product showed homology with Tnps of ISs classified as members of a distinct subgroup of the IS5 group of the IS5 family. The highest levels of similarity were observed with ISSsp126 of Sphingomonas sp. and IS1169 of Bacteroides fragilis. Analysis of the distribution of ISs of P. solventivorans revealed that ISPso2-like elements are the most widely spread of the elements in nine species of the genus Paracoccus. ISPso1 and ISPso3 are present in only a few paracoccal strains, which suggests that they were acquired by lateral transfer. Phylogenetic analysis of Tnps of the novel ISs and their closest relatives showed their evolutionary relationships and possible directions of lateral transfer between various bacterial hosts.

* Corresponding author. Mailing address: Warsaw University, Institute of Microbiology, Department of Bacterial Genetics, Miecznikowa 1, 02-096 Warsaw, Poland. Phone: 48 22 5541344. Fax: 48 22 5541404. E-mail: bartosik{at}biol.uw.edu.pl.

Applied and Environmental Microbiology, December 2003, p. 7002-7008, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7002-7008.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mikosa, M., Sochacka-Pietal, M., Baj, J., Bartosik, D. (2006). Identification of a transposable genomic island of Paracoccus pantotrophus DSM 11072 by its transposition to a novel entrapment vector pMMB2.. Microbiology 152: 1063-1073 [Abstract] [Full Text]  


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