This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dumitru, R. Articles by Ragsdale, S. W. Search for Related Content PubMed PubMed Citation Articles by Dumitru, R. Articles by Ragsdale, S. W. Agricola Articles by Dumitru, R. Articles by Ragsdale, S. W.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2003, p. 7236-7241, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7236-7241.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Targeting Methanopterin Biosynthesis To Inhibit Methanogenesis

Departments of Biochemistry,¹ Chemistry,² Animal Science, University of Nebraska-Lincoln, Lincoln, Nebraska,³ Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida⁴

Received 3 June 2003/ Accepted 1 September 2003

This paper describes the design, synthesis, and successful employment of inhibitors of 4-(ß-D-ribofuranosyl)aminobenzene-5'-phosphate (RFA-P) synthase, which catalyzes the first committed step in the biosynthesis of methanopterin, to specifically halt the growth of methane-producing microbes. RFA-P synthase catalyzes the first step in the synthesis of tetrahydromethanopterin, a key cofactor required for methane formation and for one-carbon transformations in methanogens. A number of inhibitors, which are N-substituted derivatives of p-aminobenzoic acid (pABA), have been synthesized and their inhibition constants with RFA-P synthase have been determined. Based on comparisons of the inhibition constants among various inhibitors, we propose that the pABA binding site in RFA-P synthase has a relatively large hydrophobic pocket near the amino group. These enzyme-targeted inhibitors arrest the methanogenesis and growth of pure cultures of methanogens. Supplying pABA to the culture relieves the inhibition, indicating a competitive interaction between pABA and the inhibitor at the cellular target, which is most likely RFAP synthase. The inhibitors do not adversely affect the growth of pure cultures of the bacteria (acetogens) that play a beneficial role in the rumen. Inhibitors added to dense ruminal fluid cultures (artificial rumena) halt methanogenesis; however, they do not inhibit volatile fatty acid (VFA) production and, in some cases, VFA levels are slightly elevated in the methanogenesis-inhibited cultures. We suggest that inhibiting methanopterin biosynthesis could be considered in strategies to decrease anthropogenic methane emissions, which could have an environmental benefit since methane is a potent greenhouse gas.

This article has been cited by other articles:

• Samuel, B. S., Hansen, E. E., Manchester, J. K., Coutinho, P. M., Henrissat, B., Fulton, R., Latreille, P., Kim, K., Wilson, R. K., Gordon, J. I. (2007). Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut. Proc. Natl. Acad. Sci. USA 104: 10643-10648 [Abstract] [Full Text]  
• Dumitru, R. V., Ragsdale, S. W. (2004). Mechanism of 4-({beta}-D-Ribofuranosyl)aminobenzene 5'-Phosphate Synthase, a Key Enzyme in the Methanopterin Biosynthetic Pathway. J. Biol. Chem. 279: 39389-39395 [Abstract] [Full Text]