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Applied and Environmental Microbiology, December 2003, p. 7242-7247, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7242-7247.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Transduction of Porcine Enteropathogenic Escherichia coli with a Derivative of a Shiga Toxin 2-Encoding Bacteriophage in a Porcine Ligated Ileal Loop System

István Tóth,1* Herbert Schmidt,2 Mohamed Dow,1 Anna Malik,1 Eric Oswald,3 and Béla Nagy1

Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Budapest, Hungary,1 Department of Microbiology and Hygiene, Medical Faculty Carl Gustav Carus, Technical University of Dresden, Dresden, Germany,2 ENVT-INRA, Toulouse, France3

Received 26 February 2003/ Accepted 4 September 2003

In this study, we have investigated the ability of detoxified Shiga toxin (Stx)-converting bacteriophages {Phi}3538 ({Delta}stx2::cat) (H. Schmidt et al., Appl. Environ. Microbiol. 65:3855-3861, 1999) and H-19B::Tn10d-bla (D. W. Acheson et al., Infect. Immun. 66:4496-4498, 1998) to lysogenize enteropathogenic Escherichia coli (EPEC) strains in vivo. We were able to transduce the porcine EPEC strain 1390 (O45) with {Phi}3538 ({Delta}stx2::cat) in porcine ligated ileal loops but not the human EPEC prototype strain E2348/69 (O127). Neither strain 1390 nor strain E2348/69 was lysogenized under these in vivo conditions when E. coli K-12 containing H-19B::Tn10d-bla was used as the stx1 phage donor. The repeated success in the in vivo transduction of an Stx2-encoding phage to a porcine EPEC strain in pig loops was in contrast to failures in the in vitro trials with these and other EPEC strains. These results indicate that in vivo conditions are more effective for transduction of Stx2-encoding phages than in vitro conditions.

* Corresponding author. Mailing address: Veterinary Medical Research Institute of the Hungarian Academy of Sciences, 1143 Budapest, Hungária krt. 21, Hungary. Phone: 36-1-2522455. Fax: 36-1-2521069. E-mail: tothi{at}vmri.hu.

Applied and Environmental Microbiology, December 2003, p. 7242-7247, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7242-7247.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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