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Applied and Environmental Microbiology, December 2003, p. 7517-7522, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7517-7522.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparative Sequence Analysis of the tuf and recA Genes and Restriction Fragment Length Polymorphism of the Internal Transcribed Spacer Region Sequences Supply Additional Tools for Discriminating Bifidobacterium lactis from Bifidobacterium animalis

Marco Ventura* and Ralf Zink{dagger}

Nestlé Research Center, Vers-Chez-Les-Blanc, 1000 Lausanne 26, Switzerland

Received 26 June 2003/ Accepted 9 September 2003

The relationship between Bifidobacterium lactis and Bifidobacterium animalis was examined by comparative analysis of tuf and recA gene sequences and by restriction fragment length polymorphism analysis of their internal 16S-23S transcribed spacer region sequences. The bifidobacterial strains investigated could be divided into two distinct groups within a single species based on the tuf, recA, and 16S-23S spacer region sequence analysis. Therefore, all strains of B. lactis and B. animalis could be unified as the species B. animalis and divided into two subspecies, Bifidobacterium animalis subsp. lactis and Bifidobacterium animalis subsp. animalis.

* Corresponding author. Present address: Bioscience Institute, Department of Microbiology, University College Cork, Western Road, Cork, Ireland. Phone: 41-21-7858021. Fax: 41-21-7858925. E-mail: m.ventura{at}ucc.ie.

{dagger} Present address: COGNIS Germany, 40551 Düsseldorf, Germany.

Applied and Environmental Microbiology, December 2003, p. 7517-7522, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7517-7522.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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