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Applied and Environmental Microbiology, December 2003, p. 7535-7540, Vol. 69, No. 12
0099-2240/03/$08.00+0 DOI: 10.1128/AEM.69.12.7535-7540.2003
Copyright © 2003, American
Society for
Microbiology. All Rights Reserved.
Application of Genome-Wide Expression Analysis To Identify Molecular Markers Useful in Monitoring Industrial Fermentations
Vincent J. Higgins,1,2* Peter J. Rogers,3 and Ian W. Dawes1,2
Clive
and Vera Ramaciotti Centre for Gene Function
Analysis,1
School of Biotechnology
and Biomolecular Sciences, University of New
South Wales, Sydney, New South Wales 2052,2
Carlton &
United Breweries Ltd., Abbotsford, Victoria 3067,Australia3
Received 28 July 2003/
Accepted 19 September 2003
Genome-wide
expression analysis of an industrial strain of Saccharomyces
cerevisiae identified the YOR387c and
YGL258w homologues as highly inducible in zinc-depleted
conditions. Induction was specific for zinc deficiency and was
dependent on Zap1p. The results indicate that these sequences may be
valuable molecular markers for detecting zinc deficiency in industrial
fermentations.
* Corresponding author.
Mailing address: School of Biotechnology and Biomolecular Sciences,
University of New South Wales, Sydney, New South Wales 2052, Australia.
Phone: 61 2 9385 1832. Fax: 61 2 9385 1050. E-mail:
v.higgins{at}unsw.edu.au.
Applied and Environmental Microbiology, December 2003, p. 7535-7540, Vol. 69, No. 12
0099-2240/03/$08.00+0 DOI: 10.1128/AEM.69.12.7535-7540.2003
Copyright © 2003, American
Society for
Microbiology. All Rights Reserved.
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