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Applied and Environmental Microbiology, December 2003, p. 7535-7540, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7535-7540.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Application of Genome-Wide Expression Analysis To Identify Molecular Markers Useful in Monitoring Industrial Fermentations

Vincent J. Higgins,1,2* Peter J. Rogers,3 and Ian W. Dawes1,2

Clive and Vera Ramaciotti Centre for Gene Function Analysis,1 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052,2 Carlton & United Breweries Ltd., Abbotsford, Victoria 3067,Australia3

Received 28 July 2003/ Accepted 19 September 2003

Genome-wide expression analysis of an industrial strain of Saccharomyces cerevisiae identified the YOR387c and YGL258w homologues as highly inducible in zinc-depleted conditions. Induction was specific for zinc deficiency and was dependent on Zap1p. The results indicate that these sequences may be valuable molecular markers for detecting zinc deficiency in industrial fermentations.


* Corresponding author. Mailing address: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia. Phone: 61 2 9385 1832. Fax: 61 2 9385 1050. E-mail: v.higgins{at}unsw.edu.au.


Applied and Environmental Microbiology, December 2003, p. 7535-7540, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7535-7540.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




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