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Applied and Environmental Microbiology, December 2003, p. 7549-7553, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7549-7553.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A Newly Discovered Verotoxin Variant, VT2g, Produced by Bovine Verocytotoxigenic Escherichia coli

P. H. M. Leung,1 J. S. M. Peiris,1 W. W. S. Ng,2 R. M. Robins-Browne,3 K. A. Bettelheim,3 and W. C. Yam1*

Department of Microbiology, Queen Mary Hospital,1 School of Professional and Continuing Education, The University of Hong Kong, Hong Kong SAR, People's Republic of China,2 Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia3

Received 12 May 2003/ Accepted 14 September 2003

A new verotoxin (VT) variant, designated vt2g, was identified from a bovine strain of verocytotoxigenic Escherichia coli (VTEC) serotype O2:H25. When vt2g was aligned with published sequences of vt2 and vt variants, it exhibited the highest DNA sequence homology with vt2 and vt2c. However, vt2g was not detected by vt2-specific primers and probes, although it was partially neutralized by an antiserum to the VT2A subunit. VT2g was cytotoxic for Vero and HeLa cells and was not activated by mouse intestinal mucus. The vt2g gene was detected in 3 of 409 (0.7%) bovine VTEC strains, including serotypes O2:H25, O2:H45 and Ont:H-.

* Corresponding author. Mailing address: Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong SAR, People's Republic of China. Phone: (852) 28554821. Fax: (852) 28551241. E-mail: wcyam{at}hkucc.hku.hk.

Applied and Environmental Microbiology, December 2003, p. 7549-7553, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7549-7553.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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