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Applied and Environmental Microbiology, December 2003, p. 7558-7562, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7558-7562.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Construction of a Genetically Modified Wine Yeast Strain Expressing the Aspergillus aculeatus rhaA Gene, Encoding an {alpha}-L-Rhamnosidase of Enological Interest

Paloma Manzanares,1 Margarita Orejas,1 José Vicente Gil,1 Leo H. de Graaff,2 Jaap Visser,3 and Daniel Ramón1,4*

Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (CSIC),1 Departamento de Medicina Preventiva y Salud Pública, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universitat de València, Burjassot, 46100 Valencia, Spain,4 Fungal Genomics Section, Laboratory of Microbiology, Wageningen University, NL-6703-HA,2 Fungal Genetics and Technology Consultancy, 6700-AJ Wageningen,The Netherlands3

Received 17 April 2003/ Accepted 15 September 2003

The Aspergillus aculeatus rhaA gene encoding an {alpha}-L-rhamnosidase has been expressed in both laboratory and industrial wine yeast strains. Wines produced in microvinifications, conducted using a combination of the genetically modified industrial strain expressing rhaA and another strain expressing a ß-glucosidase, show increased content mainly of the aromatic compound linalool.

* Corresponding author. Mailing address: Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Apartado Postal 73, Burjassot, 46100 Valencia, Spain. Phone: 34-96-3900022. Fax: 34-96-3636301. E-mail: daniel.ramon{at}uv.es.

Applied and Environmental Microbiology, December 2003, p. 7558-7562, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7558-7562.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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