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Applied and Environmental Microbiology, December 2003, p. 7563-7566, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7563-7566.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Genetic Characterization of the Carotenoid Biosynthetic Pathway in Methylobacterium extorquens AM1 and Isolation of a Colorless Mutant

Stephen J. Van Dien,1 Christopher J. Marx,2,{dagger} Brooke N. O'Brien,2 and Mary E. Lidstrom1,2*

Department of Chemical Engineering,1 Department of Microbiology, University of Washington, Seattle, Washington 98195-21802

Received 30 June 2003/ Accepted 22 September 2003

Genomic searches were used to reconstruct the putative carotenoid biosynthesis pathway in the pink-pigmented facultative methylotroph Methylobacterium extorquens AM1. Four genes for putative phytoene desaturases were identified. A colorless mutant was obtained by transposon mutagenesis, and the insertion was shown to be in one of the putative phytoene desaturase genes. Mutations in the other three did not affect color. The tetracycline marker was removed from the original transposon mutant, resulting in a pigment-free strain with wild-type growth properties useful as a tool for future experiments.

* Corresponding author. Mailing address: Department of Chemical Engineering, University of Washington, Box 352180, Seattle, WA 98195-2180. Phone: (206) 616-5282. Fax: (206) 616-5721. E-mail: lidstrom{at}u.washington.edu.

{dagger} Present address: 2215 Biomedical Physical Sciences, Michigan State University, East Lansing, MI 48824-4320.

Applied and Environmental Microbiology, December 2003, p. 7563-7566, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7563-7566.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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