This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ritchie, J. M.
Right arrow Articles by Waldor, M. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ritchie, J. M.
Right arrow Articles by Waldor, M. K.
Agricola
Right arrow Articles by Ritchie, J. M.
Right arrow Articles by Waldor, M. K.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2003, p. 1059-1066, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.1059-1066.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Shiga Toxin Production by Hemolytic-Uremic Syndrome-Associated and Bovine-Associated Shiga Toxin-Producing Escherichia coli Isolates

Jenny M. Ritchie,1* Patrick L. Wagner,1,2 David W. K. Acheson,3 and Matthew K. Waldor1,2

Division of Geographic Medicine and Infectious Diseases,1 Howard Hughes Medical Institute, Tufts-New England Medical Center, Boston, Massachusetts 02111,2 Department of Epidemiology and Preventive Medicine, University of Maryland, Baltimore, Maryland 212013

Received 5 September 2002/ Accepted 22 November 2002

There is considerable diversity among Shiga toxin (Stx)-producing Escherichia coli (STEC) bacteria, and only a subset of these organisms are thought to be human pathogens. The characteristics that distinguish STEC bacteria that give rise to human disease are not well understood. Stxs, the principal virulence determinants of STEC, are thought to account for hemolytic-uremic syndrome (HUS), a severe clinical consequence of STEC infection. Stxs are typically bacteriophage encoded, and their production has been shown to be enhanced by prophage-inducing agents such as mitomycin C in a limited number of clinical STEC isolates. Low iron concentrations also enhance Stx production by some clinical isolates; however, little is known regarding whether and to what extent these stimuli regulate Stx production by STEC associated with cattle, the principal environmental reservoir of STEC. In this study, we investigated whether toxin production differed between HUS- and bovine-associated STEC strains. Basal production of Stx by HUS-associated STEC exceeded that of bovine-associated STEC. In addition, following mitomycin C treatment, Stx2 production by HUS-associated STEC was significantly greater than that by bovine-associated STEC. Unexpectedly, mitomycin C treatment had a minimal effect on Stx1 production by both HUS- and bovine-associated STEC. However, Stx1 production was induced by growth in low-iron medium, and induction was more marked for HUS-associated STEC than for bovine-associated STEC. These observations reveal that disease-associated and bovine-associated STEC bacteria differ in their basal and inducible Stx production characteristics.

* Corresponding author. Mailing address: Division of Geographic Medicine and Infectious Diseases, Tufts-New England Medical Center, 750 Washington St., Boston, MA 02111. Phone: (617) 636-4134. Fax: (617) 636-5292. E-mail: jritchie1{at}lifespan.org.

Applied and Environmental Microbiology, February 2003, p. 1059-1066, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.1059-1066.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • de Sablet, T., Chassard, C., Bernalier-Donadille, A., Vareille, M., Gobert, A. P., Martin, C. (2009). Human Microbiota-Secreted Factors Inhibit Shiga Toxin Synthesis by Enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 77: 783-790 [Abstract] [Full Text]  
  • Karama, M., Johnson, R. P., Holtslander, R., Gyles, C. L. (2009). Production of Verotoxin and Distribution of O Islands 122 and 43/48 among Verotoxin-Producing Escherichia coli O103:H2 Isolates from Cattle and Humans. Appl. Environ. Microbiol. 75: 268-270 [Abstract] [Full Text]  
  • Kotewicz, M. L., Mammel, M. K., LeClerc, J. E., Cebula, T. A. (2008). Optical mapping and 454 sequencing of Escherichia coli O157 : H7 isolates linked to the US 2006 spinach-associated outbreak. Microbiology 154: 3518-3528 [Abstract] [Full Text]  
  • Karama, M., Gyles, C. L. (2008). Characterization of Verotoxin-Encoding Phages from Escherichia coli O103:H2 Strains of Bovine and Human Origins. Appl. Environ. Microbiol. 74: 5153-5158 [Abstract] [Full Text]  
  • Ziebell, K., Steele, M., Zhang, Y., Benson, A., Taboada, E. N., Laing, C., McEwen, S., Ciebin, B., Johnson, R., Gannon, V. (2008). Genotypic Characterization and Prevalence of Virulence Factors among Canadian Escherichia coli O157:H7 Strains. Appl. Environ. Microbiol. 74: 4314-4323 [Abstract] [Full Text]  
  • de Sablet, T., Bertin, Y., Vareille, M., Girardeau, J.-P., Garrivier, A., Gobert, A. P., Martin, C. (2008). Differential expression of stx2 variants in Shiga toxin-producing Escherichia coli belonging to seropathotypes A and C. Microbiology 154: 176-186 [Abstract] [Full Text]  
  • Baker, D. R., Moxley, R. A., Steele, M. B., LeJeune, J. T., Christopher-Hennings, J., Chen, D.-G., Hardwidge, P. R., Francis, D. H. (2007). Differences in Virulence among Escherichia coli O157:H7 Strains Isolated from Humans during Disease Outbreaks and from Healthy Cattle. Appl. Environ. Microbiol. 73: 7338-7346 [Abstract] [Full Text]  
  • Ochoa, T. J., Chen, J., Walker, C. M., Gonzales, E., Cleary, T. G. (2007). Rifaximin Does Not Induce Toxin Production or Phage-Mediated Lysis of Shiga Toxin-Producing Escherichia coli. Antimicrob. Agents Chemother. 51: 2837-2841 [Abstract] [Full Text]  
  • Khaitan, A., Jandhyala, D. M., Thorpe, C. M., Ritchie, J. M., Paton, A. W. (2007). The Operon Encoding SubAB, a Novel Cytotoxin, Is Present in Shiga toxin-producing Escherichia coli Isolates from the United States. J. Clin. Microbiol. 45: 1374-1375 [Full Text]  
  • Gyles, C. L. (2007). Shiga toxin-producing Escherichia coli: An overview. J ANIM SCI 85: E45-E62 [Abstract] [Full Text]  
  • Sheng, H., Lim, J. Y., Knecht, H. J., Li, J., Hovde, C. J. (2006). Role of Escherichia coli O157:H7 Virulence Factors in Colonization at the Bovine Terminal Rectal Mucosa.. Infect. Immun. 74: 4685-4693 [Abstract] [Full Text]  
  • Geue, L., Selhorst, T., Schnick, C., Mintel, B., Conraths, F. J. (2006). Analysis of the Clonal Relationship of Shiga Toxin-Producing Escherichia coli Serogroup O165:H25 Isolated from Cattle.. Appl. Environ. Microbiol. 72: 2254-2259 [Abstract] [Full Text]  
  • John, M., Kudva, I. T., Griffin, R. W., Dodson, A. W., McManus, B., Krastins, B., Sarracino, D., Progulske-Fox, A., Hillman, J. D., Handfield, M., Tarr, P. I., Calderwood, S. B. (2005). Use of In Vivo-Induced Antigen Technology for Identification of Escherichia coli O157:H7 Proteins Expressed during Human Infection. Infect. Immun. 73: 2665-2679 [Abstract] [Full Text]  
  • Maldonado, Y., Fiser, J. C., Nakatsu, C. H., Bhunia, A. K. (2005). Cytotoxicity Potential and Genotypic Characterization of Escherichia coli Isolates from Environmental and Food Sources. Appl. Environ. Microbiol. 71: 1890-1898 [Abstract] [Full Text]  
  • Ritchie, J. M., Waldor, M. K. (2005). The Locus of Enterocyte Effacement-Encoded Effector Proteins All Promote Enterohemorrhagic Escherichia coli Pathogenicity in Infant Rabbits. Infect. Immun. 73: 1466-1474 [Abstract] [Full Text]  
  • Zhang, W., Bielaszewska, M., Friedrich, A. W., Kuczius, T., Karch, H. (2005). Transcriptional Analysis of Genes Encoding Shiga Toxin 2 and Its Variants in Escherichia coli. Appl. Environ. Microbiol. 71: 558-561 [Abstract] [Full Text]  
  • Tyler, J. S., Mills, M. J., Friedman, D. I. (2004). The Operator and Early Promoter Region of the Shiga Toxin Type 2-Encoding Bacteriophage 933W and Control of Toxin Expression. J. Bacteriol. 186: 7670-7679 [Abstract] [Full Text]  
  • De Baets, L., Van der Taelen, I., De Filette, M., Pierard, D., Allison, L., De Greve, H., Hernalsteens, J.-P., Imberechts, H. (2004). Genetic Typing of Shiga Toxin 2 Variants of Escherichia coli by PCR-Restriction Fragment Length Polymorphism Analysis. Appl. Environ. Microbiol. 70: 6309-6314 [Abstract] [Full Text]  
  • Brussow, H., Canchaya, C., Hardt, W.-D. (2004). Phages and the Evolution of Bacterial Pathogens: from Genomic Rearrangements to Lysogenic Conversion. Microbiol. Mol. Biol. Rev. 68: 560-602 [Abstract] [Full Text]  
  • Muniesa, M., Blanco, J. E., de Simon, M., Serra-Moreno, R., Blanch, A. R., Jofre, J. (2004). Diversity of stx2 converting bacteriophages induced from Shiga-toxin-producing Escherichia coli strains isolated from cattle. Microbiology 150: 2959-2971 [Abstract] [Full Text]  
  • Djordjevic, S. P., Ramachandran, V., Bettelheim, K. A., Vanselow, B. A., Holst, P., Bailey, G., Hornitzky, M. A. (2004). Serotypes and Virulence Gene Profiles of Shiga Toxin-Producing Escherichia coli Strains Isolated from Feces of Pasture-Fed and Lot-Fed Sheep. Appl. Environ. Microbiol. 70: 3910-3917 [Abstract] [Full Text]  
  • Chibani-Chennoufi, S., Bruttin, A., Dillmann, M.-L., Brussow, H. (2004). Phage-Host Interaction: an Ecological Perspective. J. Bacteriol. 186: 3677-3686 [Full Text]  
  • Ritchie, J. M., Thorpe, C. M., Rogers, A. B., Waldor, M. K. (2003). Critical Roles for stx2, eae, and tir in Enterohemorrhagic Escherichia coli-Induced Diarrhea and Intestinal Inflammation in Infant Rabbits. Infect. Immun. 71: 7129-7139 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»