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Applied and Environmental Microbiology, February 2003, p. 1315-1319, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.1315-1319.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Genetic Differences between Two Strains of Xylella fastidiosa Revealed by Suppression Subtractive Hybridization{dagger}

Ricardo Harakava{ddagger} and Dean W. Gabriel*

Department of Plant Pathology, University of Florida, Gainesville, Florida 32611

Received 11 July 2002/ Accepted 2 November 2002

Suppression subtractive hybridization was used to rapidly identify 18 gene differences between a citrus variegated chlorosis (CVC) strain and a Pierce's disease of grape (PD) strain of Xylella fastidiosa. The results were validated as being highly representative of actual differences by comparison of the completely sequenced genome of a CVC strain with that of a PD strain.

* Corresponding author. Mailing address: Department of Plant Pathology, University of Florida, 1453 Fifield Hall, P.O. Box 110680, Gainesville, FL 32611-0680. Phone: (352) 392-7239. Fax: (352) 392-6532. E-mail: gabriel{at}biotech.ufl.edu.

{dagger} Florida Agricultural Experiment Station contribution no. R09260.

{ddagger} Present address: Instituto Biológico, CP 12898, São Paulo 04010-370, Brazil.

Applied and Environmental Microbiology, February 2003, p. 1315-1319, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.1315-1319.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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