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Applied and Environmental Microbiology, February 2003, p. 1320-1324, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.1320-1324.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Dibenzyl Sulfide Metabolism by White Rot Fungi

Jonathan D. Van Hamme,1* Eddie T. Wong,1 Heather Dettman,1 Murray R. Gray,2 and Michael A. Pickard3

National Centre for Upgrading Technology, Devon, Alberta T9G 1A8,1 Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta T6G 2G6,2 Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada3

Received 15 July 2002/ Accepted 6 November 2002

Microbial metabolism of organosulfur compounds is of interest in the petroleum industry for in-field viscosity reduction and desulfurization. Here, dibenzyl sulfide (DBS) metabolism in white rot fungi was studied. Trametes trogii UAMH 8156, Trametes hirsuta UAMH 8165, Phanerochaete chrysosporium ATCC 24725, Trametes versicolor IFO 30340 (formerly Coriolus sp.), and Tyromyces palustris IFO 30339 all oxidized DBS to dibenzyl sulfoxide prior to oxidation to dibenzyl sulfone. The cytochrome P-450 inhibitor 1-aminobenzotriazole eliminated dibenzyl sulfoxide oxidation. Laccase activity (0.15 U/ml) was detected in the Trametes cultures, and concentrated culture supernatant and pure laccase catalyzed DBS oxidation to dibenzyl sulfoxide more efficiently in the presence of 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) than in its absence. These data suggest that the first oxidation step is catalyzed by extracellular enzymes but that subsequent metabolism is cytochrome P-450 mediated.

* Corresponding author. Mailing address: National Centre for Upgrading Technology, 1 Oil Patch Dr., Suite A202, Edmonton, Alberta T9G 1A8, Canada. Phone: (708) 987-8752. Fax: (780) 987-5349. E-mail: jvanhamme{at}alumni.uwaterloo.ca.

Applied and Environmental Microbiology, February 2003, p. 1320-1324, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.1320-1324.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Van Hamme, J. D., Fedorak, P. M., Foght, J. M., Gray, M. R., Dettman, H. D. (2004). Use of a Novel Fluorinated Organosulfur Compound To Isolate Bacteria Capable of Carbon-Sulfur Bond Cleavage. Appl. Environ. Microbiol. 70: 1487-1493 [Abstract] [Full Text]  


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