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Applied and Environmental Microbiology, February 2003, p. 1325-1326, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.1325-1326.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Chlorine Inactivation of Spores of Encephalitozoon spp.

C. H. Johnson,1* M. M. Marshall,2 L. A. DeMaria,1 J. M. Moffet,2 and D. G. Korich2

U.S. Environmental Protection Agency, Cincinnati, Ohio 45268,1 Sterling Parasitology Laboratory, University of Arizona, Tucson, Arizona 857212

Received 27 February 2002/ Accepted 13 November 2002

This report is an extension of a preliminary investigation on the use of chlorine to inactivate spores of Encephalitozoon intestinalis and to investigate the effect of chlorine on two other species, E cuniculi and E. hellem, associated with human infection. The 50% tissue culture infective doses of these three species were also determined. On the basis of the results obtained, it appears that chlorination of water is an effective means of controlling spores of these organisms in the aquatic environment.


* Corresponding author. Mailing address: U.S. Environmental Protection Agency, 26 W. M. L. King Dr., MS-387, Cincinnati, OH 45268. Phone: (513) 569-7345. Fax: (513) 569-7328. E-mail: johnson.cliff{at}epa.gov.


Applied and Environmental Microbiology, February 2003, p. 1325-1326, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.1325-1326.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




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