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Applied and Environmental Microbiology, February 2003, p. 1331-1334, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.1331-1334.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Recombinant Strain of Bacillus thuringiensis Producing Cyt1A, Cry11B, and the Bacillus sphaericus Binary Toxin

Hyun-Woo Park,1 Dennis K. Bideshi,1 and Brian A. Federici1,2*

Department of Entomology,1 Interdepartmental Graduate Programs in Genetics and Microbiology, University of California, Riverside, California 925212

Received 19 August 2002/ Accepted 5 November 2002

A novel recombinant Bacillus thuringiensis subsp. israelensis strain that produces the B. sphaericus binary toxin, Cyt1Aa, and Cry11Ba is described. The toxicity of this strain (50% lethal concentration [LC50] = 1.7 ng/ml) against fourth-instar Culex quinquefasciatus was higher than that of B. thuringiensis subsp. israelensis IPS-82 (LC50 = 7.9 ng/ml) or B. sphaericus 2362 (LC50 = 12.6 ng/ml).


* Corresponding author. Mailing address: Department of Entomology and Graduate Genetics Program, University of California, Riverside, California 92521. Phone: (909) 787-5006. Fax: (909) 787-3086. E-mail: brian.federici{at}ucr.edu.


Applied and Environmental Microbiology, February 2003, p. 1331-1334, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.1331-1334.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




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