This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Inoue, H. Articles by Yamaoka, Y. Search for Related Content PubMed PubMed Citation Articles by Inoue, H. Articles by Yamaoka, Y. Agricola Articles by Inoue, H. Articles by Yamaoka, Y.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2003, p. 878-883, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.878-883.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Tin-Carbon Cleavage of Organotin Compounds by Pyoverdine from Pseudomonas chlororaphis

Hiroyuki Inoue,^1* Osamu Takimura,¹ Ken Kawaguchi,² Teruhiko Nitoda,³ Hiroyuki Fuse,¹ Katsuji Murakami,¹ and Yukiho Yamaoka¹

Institute for Marine Resources and Environment, National Institute of Advanced Industrial Science and Technology, Hiroshima 737-0197,¹ Analytical Science Department, Toray Research Center, Inc., Kanagawa 248-8555 ,² Department of Biological Function, Faculty of Agriculture, Okayama University, Okayama 700-8530, Japan³

Received 19 July 2002/ Accepted 19 November 2002

The triphenyltin (TPT)-degrading bacterium Pseudomonas chlororaphis CNR15 produces extracellular yellow substances to degrade TPT. Three substances (F-I, F-IIa, and F-IIb) were purified, and their structural and catalytic properties were characterized. The primary structure of F-I was established using two-dimensional nuclear magnetic resonance techniques; the structure was identical to that of suc-pyoverdine from P. chlororaphis ATCC 9446, which is a peptide siderophore produced by fluorescent pseudomonads. Spectral and isoelectric-focusing analyses revealed that F-IIa and F-IIb were also pyoverdines, differing only in the acyl substituent attached to the chromophore part of F-I. Furthermore, we found that the fluorescent pseudomonads producing pyoverdines structurally different from F-I showed TPT degradation activity in the solid extracts of their culture supernatants. F-I and F-IIa degraded TPT to monophenyltin via diphenyltin (DPT) and degraded DPT and dibutyltin to monophenyltin and monobutyltin, respectively. The total amount of organotin metabolites produced by TPT degradation was nearly equivalent to that of the F-I added to the reaction mixture, whereas DPT degradation was not influenced by monophenyltin production. The TPT degradation activity of F-I was remarkably inhibited by the addition of metal ions chelated with pyoverdine. On the other hand, the activity of DPT was increased 13- and 8-fold by the addition of Cu²⁺ and Sn⁴⁺, respectively. These results suggest that metal-chelating ligands common to pyoverdines may play important roles in the Sn-C cleavage of organotin compounds in both the metal-free and metal-complexed states.

---

* Corresponding author. Mailing address: Institute for Marine Resources and Environment, National Institute of Advanced Industrial Science and Technology, 2-2-2 Hiro-Suehiro, Kure, Hiroshima 737-0197, Japan. Phone: 81-823-72-1935. Fax: 81-823-73-3284. E-mail:inoue-h{at}aist.go.jp.

---

Applied and Environmental Microbiology, February 2003, p. 878-883, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.878-883.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Sun, G.-X., Zhong, J.-J. (2006). Mechanism of Augmentation of Organotin Decomposition by Ferripyochelin: Formation of Hydroxyl Radical and Organotin-Pyochelin-Iron Ternary Complex. Appl. Environ. Microbiol. 72: 7264-7269 [Abstract] [Full Text]  
• Sun, G.-X., Zhou, W.-Q., Zhong, J.-J. (2006). Organotin Decomposition by Pyochelin, Secreted by Pseudomonas aeruginosa Even in an Iron-Sufficient Environment. Appl. Environ. Microbiol. 72: 6411-6413 [Abstract] [Full Text]