This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suzuki, Y.
Right arrow Articles by Banfield, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suzuki, Y.
Right arrow Articles by Banfield, J. F.
Agricola
Right arrow Articles by Suzuki, Y.
Right arrow Articles by Banfield, J. F.

Next Article 

Applied and Environmental Microbiology, March 2003, p. 1337-1346, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1337-1346.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Microbial Populations Stimulated for Hexavalent Uranium Reduction in Uranium Mine Sediment

Yohey Suzuki,1* Shelly D. Kelly,2 Kenneth M. Kemner,2 and Jillian F. Banfield3

Department of Geology and Geophysics, University of Wisconsin—Madison, Madison, Wisconsin 53706,1 Environmental Research Division, Argonne National Laboratory, Argonne, Illinois 60439,2 Department of Earth and Planetary Sciences, University of California—Berkeley, Berkeley, California 947203

Received 23 May 2002/ Accepted 6 September 2002

Uranium-contaminated sediment and water collected from an inactive uranium mine were incubated anaerobically with organic substrates. Stimulated microbial populations removed U almost entirely from solution within 1 month. X-ray absorption near-edge structure analysis showed that U(VI) was reduced to U(IV) during the incubation. Observations by transmission electron microscopy, selected area diffraction pattern analysis, and energy-dispersive X-ray spectroscopic analysis showed two distinct types of prokaryotic cells that precipitated only a U(IV) mineral uraninite (UO2) or both uraninite and metal sulfides. Prokaryotic cells associated with uraninite and metal sulfides were inferred to be sulfate-reducing bacteria. Phylogenetic analysis of 16S ribosomal DNA obtained from the original and incubated sediments revealed that microbial populations were changed from microaerophilic Proteobacteria to anaerobic low-G+C gram-positive sporeforming bacteria by the incubation. Forty-two out of 94 clones from the incubated sediment were related to sulfate-reducing Desulfosporosinus spp., and 23 were related to fermentative Clostridium spp. The results suggest that, if in situ bioremediation were attempted in the uranium mine ponds, Desulfosporosinus spp. would be a major contributor to U(VI) and sulfate reduction and Clostridium spp. to U(VI) reduction.

* Corresponding author. Present address: Frontier Research System for Extremophiles, Japan Marine Science & Technology Center, 2-15, Natushima-cho, Yokosuka 237-006, Japan. Phone: 81(468)67 9710. Fax: 81(468)67 9715. E-mail: yohey{at}jamstec.go.jp.

Applied and Environmental Microbiology, March 2003, p. 1337-1346, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1337-1346.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Madden, A. S., Palumbo, A. V., Ravel, B., Vishnivetskaya, T. A., Phelps, T. J., Schadt, C. W., Brandt, C. C. (2009). Donor-dependent Extent of Uranium Reduction for Bioremediation of Contaminated Sediment Microcosms. J. Environ. Qual. 38: 53-60 [Abstract] [Full Text]  
  • Gao, W., Francis, A. J. (2008). Reduction of Uranium(VI) to Uranium(IV) by Clostridia. Appl. Environ. Microbiol. 74: 4580-4584 [Abstract] [Full Text]  
  • Cardenas, E., Wu, W.-M., Leigh, M. B., Carley, J., Carroll, S., Gentry, T., Luo, J., Watson, D., Gu, B., Ginder-Vogel, M., Kitanidis, P. K., Jardine, P. M., Zhou, J., Criddle, C. S., Marsh, T. L., Tiedje, J. M. (2008). Microbial Communities in Contaminated Sediments, Associated with Bioremediation of Uranium to Submicromolar Levels. Appl. Environ. Microbiol. 74: 3718-3729 [Abstract] [Full Text]  
  • McKinley, J. P., Zachara, J. M., Wan, J., McCready, D. E., Heald, S. M. (2007). Geochemical Controls on Contaminant Uranium in Vadose Hanford Formation Sediments at the 200 Area and 300 Area, Hanford Site, Washington. Vadose Zone J 6: 1004-1017 [Abstract] [Full Text]  
  • Spain, A. M., Peacock, A. D., Istok, J. D., Elshahed, M. S., Najar, F. Z., Roe, B. A., White, D. C., Krumholz, L. R. (2007). Identification and Isolation of a Castellaniella Species Important during Biostimulation of an Acidic Nitrate- and Uranium-Contaminated Aquifer. Appl. Environ. Microbiol. 73: 4892-4904 [Abstract] [Full Text]  
  • Ramamoorthy, S., Sass, H., Langner, H., Schumann, P., Kroppenstedt, R. M., Spring, S., Overmann, J., Rosenzweig, R. F. (2006). Desulfosporosinus lacus sp. nov., a sulfate-reducing bacterium isolated from pristine freshwater lake sediments. Int. J. Syst. Evol. Microbiol. 56: 2729-2736 [Abstract] [Full Text]  
  • Brodie, E. L., DeSantis, T. Z., Joyner, D. C., Baek, S. M., Larsen, J. T., Andersen, G. L., Hazen, T. C., Richardson, P. M., Herman, D. J., Tokunaga, T. K., Wan, J. M., Firestone, M. K. (2006). Application of a High-Density Oligonucleotide Microarray Approach To Study Bacterial Population Dynamics during Uranium Reduction and Reoxidation. Appl. Environ. Microbiol. 72: 6288-6298 [Abstract] [Full Text]  
  • Moon, J.-W., Roh, Y., Phelps, T. J., Phillips, D. H., Watson, D. B., Kim, Y.-J., Brooks, S. C. (2006). Physicochemical and Mineralogical Characterization of Soil-Saprolite Cores from a Field Research Site, Tennessee. J. Environ. Qual. 35: 1731-1741 [Abstract] [Full Text]  
  • Beller, H. R., Chain, P. S. G., Letain, T. E., Chakicherla, A., Larimer, F. W., Richardson, P. M., Coleman, M. A., Wood, A. P., Kelly, D. P. (2006). The Genome Sequence of the Obligately Chemolithoautotrophic, Facultatively Anaerobic Bacterium Thiobacillus denitrificans. J. Bacteriol. 188: 1473-1488 [Abstract] [Full Text]  
  • Hu, P., Brodie, E. L., Suzuki, Y., McAdams, H. H., Andersen, G. L. (2005). Whole-Genome Transcriptional Analysis of Heavy Metal Stresses in Caulobacter crescentus. J. Bacteriol. 187: 8437-8449 [Abstract] [Full Text]  
  • Suzuki, Y., Sato, T., Isobe, H., Kogure, T., Murakami, T. (2005). Dehydration processes in the meta-autunite group minerals meta-autunite, metasaleeite, and metatorbernite. American Mineralogist 90: 1308-1314 [Abstract] [Full Text]  
  • Suzuki, Y., Kelly, S. D., Kemner, K. M., Banfield, J. F. (2005). Direct Microbial Reduction and Subsequent Preservation of Uranium in Natural Near-Surface Sediment. Appl. Environ. Microbiol. 71: 1790-1797 [Abstract] [Full Text]  
  • Beller, H. R. (2005). Anaerobic, Nitrate-Dependent Oxidation of U(IV) Oxide Minerals by the Chemolithoautotrophic Bacterium Thiobacillus denitrificans. Appl. Environ. Microbiol. 71: 2170-2174 [Abstract] [Full Text]  
  • North, N. N., Dollhopf, S. L., Petrie, L., Istok, J. D., Balkwill, D. L., Kostka, J. E. (2004). Change in Bacterial Community Structure during In Situ Biostimulation of Subsurface Sediment Cocontaminated with Uranium and Nitrate. Appl. Environ. Microbiol. 70: 4911-4920 [Abstract] [Full Text]  
  • Anderson, R. T., Vrionis, H. A., Ortiz-Bernad, I., Resch, C. T., Long, P. E., Dayvault, R., Karp, K., Marutzky, S., Metzler, D. R., Peacock, A., White, D. C., Lowe, M., Lovley, D. R. (2003). Stimulating the In Situ Activity of Geobacter Species To Remove Uranium from the Groundwater of a Uranium-Contaminated Aquifer. Appl. Environ. Microbiol. 69: 5884-5891 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»