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Applied and Environmental Microbiology, March 2003, p. 1581-1588, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1581-1588.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Conidial Hydrophobins of Aspergillus fumigatus

Sophie Paris,1* Jean-Paul Debeaupuis,1 Reto Crameri,2 Marilyn Carey,3 Franck Charlès,4 Marie Christine Prévost,5 Christine Schmitt,5 Bruno Philippe,1 and Jean Paul Latgé1

Unité des Aspergillus, Département Structure et Dynamique des génomes,1 Plateforme de Microscopie électronique, Institut Pasteur, Paris,5 Jeol Europe, Croissy-sur-Seine, France,4 Swiss Institute of Allergy and Asthma Research, Davos, Switzerland,2 Gatan, Oxford, United Kingdom3

Received 17 September 2002/ Accepted 28 November 2002

The surface of Aspergillus fumigatus conidia, the first structure recognized by the host immune system, is covered by rodlets. We report that this outer cell wall layer contains two hydrophobins, RodAp and RodBp, which are found as highly insoluble complexes. The RODA gene was previously characterized, and {Delta}rodA conidia do not display a rodlet layer (N. Thau, M. Monod, B. Crestani, C. Rolland, G. Tronchin, J. P. Latgé, and S. Paris, Infect. Immun. 62:4380-4388, 1994). The RODB gene was cloned and disrupted. RodBp was highly homologous to RodAp and different from DewAp of A. nidulans. {Delta}rodB conidia had a rodlet layer similar to that of the wild-type conidia. Therefore, unlike RodAp, RodBp is not required for rodlet formation. The surface of {Delta}rodA conidia is granular; in contrast, an amorphous layer is present at the surface of the conidia of the {Delta}rodA {Delta}rodB double mutant. These data show that RodBp plays a role in the structure of the conidial cell wall. Moreover, rodletless mutants are more sensitive to killing by alveolar macrophages, suggesting that RodAp or the rodlet structure is involved in the resistance to host cells.

* Corresponding author. Mailing address: Unité des Aspergillus, Institut Pasteur, 25 rue du Docteur Roux, F-75724 Paris Cedex 15, France. Phone: 33 1 45 68 82 25. Fax: 33 1 40 61 34 19. E-mail: sparis{at}pasteur.fr.

Applied and Environmental Microbiology, March 2003, p. 1581-1588, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1581-1588.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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