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Applied and Environmental Microbiology, March 2003, p. 1642-1646, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1642-1646.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Enterotoxigenicity and Genetic Relatedness of Clostridium perfringens Isolates from Retail Foods in the United States

Yuan-Tong Lin and Ronald Labbe^*

Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003

Received 10 June 2002/ Accepted 29 November 2002

Clostridium perfringens is a leading cause of bacterial food-borne illness in countries where consumption of meat and poultry is high. For example, each year in the United States, this organism is the second or third most common cause of confirmed cases of food-borne illness. Surveys of the incidence of this organism in retail foods were done in the 1960s without regard to whether isolates were enterotoxigenic. It is now known that not all strains of this organism possess the enterotoxin gene responsible for illness. We examined the incidence of this organism in 131 food samples from retail food stores in an area of the northeastern United States. Forty isolates were obtained by using the iron milk method at 45°C, with confirmation by use of motility nitrate and lactose gelatin media. The presence of the C. perfringens enterotoxin (cpe) and alpha toxin (cpa) genes was determined by PCR using previously published primer sequences. All isolates possessed cpa. None of the isolates were identified as carrying the cpe gene by this method or by another method using a digoxigenin-labeled gene probe. Consistent with these results, none of the sporulating-cell extracts contained enterotoxin as determined by reverse passive latex hemagglutination. Pulsed-field gel electrophoresis was used to determine the genetic relatedness of the isolates. About 5% of the isolates were considered to be closely related (2- to 3-band difference). The others were considered to be unrelated to one another. The results demonstrate the rarity of cpe⁺ strains in retail foods and the genetic diversity among nonoutbreak strains.

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* Corresponding author. Mailing address: Department of Food Science, Chenoweth Laboratory, University of Massachusetts, Amherst, MA 01003-9285. Phone: (413) 545-1021. Fax: (413) 545-1262. E-mail: rlabbe{at}foodsci.umass.edu.

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Applied and Environmental Microbiology, March 2003, p. 1642-1646, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1642-1646.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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