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Applied and Environmental Microbiology, March 2003, p. 1687-1694, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1687-1694.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Prevalence of Bacteria of Division TM7 in Human Subgingival Plaque and Their Association with Disease

Mary M. Brinig,1,2* Paul W. Lepp,1,2,3 Cleber C. Ouverney,1,2,3 Gary C. Armitage,4 and David A. Relman1,2,3

Departments of Microbiology and Immunology,1 Medicine, Stanford University, Stanford,3 VA Palo Alto Health Care System, Palo Alto,2 Department of Stomatology, University of California, San Francisco, California4

Received 4 September 2002/ Accepted 13 December 2002

Members of the uncultivated bacterial division TM7 have been detected in the human mouth, but little information is available regarding their prevalence and diversity at this site. Human subgingival plaque samples from healthy sites and sites exhibiting various stages of periodontal disease were analyzed for the presence of TM7 bacteria. TM7 ribosomal DNA (rDNA) was found in 96% of the samples, and it accounted for approximately 0.3%, on average, of all bacterial rDNA in the samples as determined by real-time quantitative PCR. Two new phylotypes of this division were identified, and members of the division were found to exhibit filamentous morphology by fluorescence in situ hybridization. The abundance of TM7 rDNA relative to total bacterial rDNA was higher in sites with mild periodontitis (0.54% ± 0.1%) than in either healthy sites (0.21% ± 0.05%, P < 0.01) or sites with severe periodontitis (0.29% ± 0.06%, P < 0.05). One division subgroup, the I025 phylotype, was detected in 1 of 18 healthy samples and 38 of 58 disease samples. These data suggest that this phylotype, and the TM7 bacterial division in general, may play a role in the multifactorial process leading to periodontitis.

* Corresponding author. Mailing address: VA Palo Alto Health Care System 154T, Building 101, Room B4-185, 3801 Miranda Ave., Palo Alto, CA 94304. Phone: (650) 493-5000, ext. 63193. Fax: (650) 852-3291. E-mail: mbrinig{at}stanford.edu.

Applied and Environmental Microbiology, March 2003, p. 1687-1694, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1687-1694.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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