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Applied and Environmental Microbiology, March 2003, p. 1836-1839, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1836-1839.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Ribotyping and Repetitive Extragenic Palindromic-PCR for Identification of Fecal Escherichia coli from Humans and Animals

C. Andrew Carson,1* Brian L. Shear,1 Mark R. Ellersieck,2 and Jennifer D. Schnell1

Department of Veterinary Pathobiology,1 Agricultural Experiment Station Statisticians, University of Missouri, Columbia, Missouri 652112

Received 29 July 2002/ Accepted 18 December 2002

This report compares the performances of two popular genotypic methods used for tracking the sources of fecal pollution in water, ribotyping and repetitive extragenic palindromic-PCR (rep-PCR). The rep-PCR was more accurate, reproducible, and efficient in associating DNA fingerprints of fecal Escherichia coli with human and animal hosts of origin.

* Corresponding author. Mailing address: 201 Connaway Hall, University of Missouri, Columbia, MO 65211. Phone: (573) 884-7640. Fax: (573) 884-0521. E-mail: carsonc{at}missouri.edu.

Applied and Environmental Microbiology, March 2003, p. 1836-1839, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1836-1839.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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