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Applied and Environmental Microbiology, March 2003, p. 1844-1846, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1844-1846.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Methods for DNA Isolation from Food Samples for Detection of Shiga Toxin-Producing Escherichia coli by Real-Time PCR

Loree C. Heller,1* Carisa R. Davis,2 K. Kealy Peak,2 David Wingfield,3 Andrew C. Cannons,2 Philip T. Amuso,2,3 and Jacqueline Cattani2

Esoteric Testing, Department of Pathology, Tampa General Hospital, Tampa, Florida 33101,1 Center for Biological Defense, University of South Florida College of Public Health,2 Bureau of Laboratories—Tampa, Florida Department of Health, Tampa, Florida 336123

Received 21 June 2002/ Accepted 29 November 2002

In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

* Corresponding author. Mailing address: Tampa General Hospital, P.O. Box 1289, Tampa, FL 33101. Phone: (813) 844-4677. Fax: (813) 844-1312. E-mail: lheller{at}tgh.org.

Applied and Environmental Microbiology, March 2003, p. 1844-1846, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1844-1846.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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