AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sata, S.
Right arrow Articles by Shimada, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sata, S.
Right arrow Articles by Shimada, T.
Agricola
Right arrow Articles by Sata, S.
Right arrow Articles by Shimada, T.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2003, p. 1858-1860, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1858-1860.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

An Improved Enrichment Broth for Isolation of Escherichia coli O157, with Specific Reference to Starved Cells, from Radish Sprouts

Shin Sata,1 Tomohiko Fujisawa,1 Ro Osawa,2* Atsushi Iguchi,2 Shiro Yamai,1 and Toshio Shimada3

Kanagawa Prefectural Public Health Laboratory, Asahi-ku, Yokohama 241-0815,1 Department of Bioscience, Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe 657-8501,2 Department of Bacteriology, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo 162-8670, Japan3

Received 8 July 2002/ Accepted 13 December 2002

An enrichment broth was developed for the efficient isolation of Escherichia coli O157 from radish sprouts. The broth was buffered peptone water containing 0.5% sodium thioglycolate (STG-BPW), which was designed to allow growth of E. coli O157 in starved and unstarved states. However, this medium suppressed the growth of non-carbohydrate-fermenting obligate aerobes whose colonial appearance on sorbitol MacConkey agar containing cefixime and tellurite (CT-SMAC) resembled that of E. coli O157. Both starved and unstarved cells of E. coli O157 experimentally inoculated into radish sprouts were successfully recovered with STG-BPW enrichment in all cases, most of which showed marked disappearance of E. coli O157-like colonies on CT-SMAC.

* Corresponding author. Mailing address: Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe 657-8501, Japan. Phone: 81 78 803 5804. Fax: 81 78 803 5804. E-mail: osawa{at}ans.kobe-u.ac.jp.

Applied and Environmental Microbiology, March 2003, p. 1858-1860, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1858-1860.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2003 by the American Society for Microbiology. All rights reserved.