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Applied and Environmental Microbiology, April 2003, p. 1904-1912, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.1904-1912.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Colonization of Vitis vinifera by a Green Fluorescence Protein-Labeled, gfp-Marked Strain of Xylophilus ampelinus, the Causal Agent of Bacterial Necrosis of Grapevine

Sophie Grall and Charles Manceau*

UMR Pathologie Végétale, INRA-INH-Université d'Angers, Institut National de la Recherche Agronomique, Centre d'Angers, F-49071 Beaucouzé, France

Received 15 August 2002/ Accepted 9 January 2003

The dynamics of Xylophilus ampelinus were studied in Vitis vinifera cv. Ugni blanc using gfp-marked bacterial strains to evaluate the relative importance of epiphytic and endophytic phases of plant colonization in disease development. Currently, bacterial necrosis of grapevine is of economic importance in vineyards in three regions in France: the Cognac, Armagnac, and Die areas. This disease is responsible for progressive destruction of vine shoots, leading to their death. We constructed gfp-marked strains of the CFBP2098 strain of X. ampelinus for histological studies. We studied the colonization of young plants of V. vinifera cv. Ugni blanc by X. ampelinus after three types of artificial contamination in a growth chamber and in a greenhouse. (i) After wounding of the stem and inoculation, the bacteria progressed down to the crown through the xylem vessels, where they organized into biofilms. (ii) When the bacteria were forced into woody cuttings, they rarely colonized the emerging plantlets. Xylem vessels could play a key role in the multiplication and conservation of the bacteria, rather than being a route for plant colonization. (iii) When bacterial suspensions were sprayed onto the plants, bacteria progressed in two directions: both in emerging organs and down to the crown, thus displaying the importance of epiphytic colonization in disease development.

* Corresponding author. Mailing address: UMR Pathologie Végétale, INRA-INH-Université d'Angers, Institut National de la Recherche Agronomique, Centre d'Angers, 42 rue Georges Morel, F-49071 Beaucouzé, France. Phone: (33) 241 22 57 17. Fax: (33) 241 22 57 05. E-mail: manceau{at}angers.inra.fr.

Applied and Environmental Microbiology, April 2003, p. 1904-1912, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.1904-1912.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Grall, S., Roulland, C., Guillaumes, J., Manceau, C. (2005). Bleeding Sap and Old Wood Are the Two Main Sources of Contamination of Merging Organs of Vine Plants by Xylophilus ampelinus, the Causal Agent of Bacterial Necrosis. Appl. Environ. Microbiol. 71: 8292-8300 [Abstract] [Full Text]  


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