This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Walter, J.
Right arrow Articles by Hertel, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Walter, J.
Right arrow Articles by Hertel, C.
Agricola
Right arrow Articles by Walter, J.
Right arrow Articles by Hertel, C.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, April 2003, p. 2044-2051, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2044-2051.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of Lactobacillus reuteri Genes Specifically Induced in the Mouse Gastrointestinal Tract

Jens Walter,1,2 Nicholas C. K. Heng,2 Walter P. Hammes,1 Diane M. Loach,2 Gerald W. Tannock,2 and Christian Hertel1*

Institute of Food Technology, University of Hohenheim, Stuttgart, Germany,1 Department of Microbiology, University of Otago, Dunedin, New Zealand2

Received 12 September 2002/ Accepted 16 January 2003

Lactobacilli are common inhabitants of the gastrointestinal tracts of mammals and have received considerable attention due to their putative health-promoting properties. Little is known about the traits that enhance the ability of these bacteria to inhabit the gastrointestinal tract. In this paper we describe the development and application of a strategy based on in vivo expression technology (IVET) that enables detection of Lactobacillus reuteri genes specifically induced in the murine gut. A plasmid-based system was constructed containing 'ermGT (which confers lincomycin resistance) as the primary reporter gene for selection of promoters active in the gastrointestinal tract of mice treated with lincomycin. A second reporter gene, 'bglM (ß-glucanase), allowed differentiation between constitutive and in vivo inducible promoters. The system was successfully tested in vitro and in vivo by using a constitutive promoter. Application of the IVET system with chromosomal DNA of L. reuteri 100-23 and reconstituted lactobacillus-free mice revealed three genes induced specifically during colonization. Two of the sequences showed homology to genes encoding xylose isomerase (xylA) and peptide methionine sulfoxide reductase (msrB), which are involved in nutrient acquisition and stress responses, respectively. The third locus showed homology to the gene encoding a protein whose function is not known. Our IVET system has the potential to identify genes of lactobacilli that have not previously been functionally characterized but which may be essential for growth of these bacteria in the gastrointestinal ecosystem.

* Corresponding author. Mailing address: Institute of Food Technology, University of Hohenheim, Garbenstr. 28, D-70599 Stuttgart, Germany. Phone: 49 711 459 4255. Fax: 49 711 459 4199. E-mail: hertel{at}uni-hohenheim.de.

Applied and Environmental Microbiology, April 2003, p. 2044-2051, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2044-2051.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Santos, F., Teusink, B., Molenaar, D., van Heck, M., Wels, M., Sieuwerts, S., de Vos, W. M., Hugenholtz, J. (2009). Effect of Amino Acid Availability on Vitamin B12 Production in Lactobacillus reuteri. Appl. Environ. Microbiol. 75: 3930-3936 [Abstract] [Full Text]  
  • Lebeer, S., Vanderleyden, J., De Keersmaecker, S. C. J. (2008). Genes and Molecules of Lactobacilli Supporting Probiotic Action. Microbiol. Mol. Biol. Rev. 72: 728-764 [Abstract] [Full Text]  
  • Denou, E., Pridmore, R. D., Ventura, M., Pittet, A.-C., Zwahlen, M.-C., Berger, B., Barretto, C., Panoff, J.-M., Brussow, H. (2008). The Role of Prophage for Genome Diversification within a Clonal Lineage of Lactobacillus johnsonii: Characterization of the Defective Prophage LJ771. J. Bacteriol. 190: 5806-5813 [Abstract] [Full Text]  
  • Walter, J. (2008). Ecological Role of Lactobacilli in the Gastrointestinal Tract: Implications for Fundamental and Biomedical Research. Appl. Environ. Microbiol. 74: 4985-4996 [Full Text]  
  • Lebeer, S., Claes, I. J. J., Verhoeven, T. L. A., Shen, C., Lambrichts, I., Ceuppens, J. L., Vanderleyden, J., De Keersmaecker, S. C. J. (2008). Impact of luxS and Suppressor Mutations on the Gastrointestinal Transit of Lactobacillus rhamnosus GG. Appl. Environ. Microbiol. 74: 4711-4718 [Abstract] [Full Text]  
  • Santos, F., Wegkamp, A., de Vos, W. M., Smid, E. J., Hugenholtz, J. (2008). High-Level Folate Production in Fermented Foods by the B12 Producer Lactobacillus reuteri JCM1112. Appl. Environ. Microbiol. 74: 3291-3294 [Abstract] [Full Text]  
  • Denou, E., Pridmore, R. D., Berger, B., Panoff, J.-M., Arigoni, F., Brussow, H. (2008). Identification of Genes Associated with the Long-Gut-Persistence Phenotype of the Probiotic Lactobacillus johnsonii Strain NCC533 Using a Combination of Genomics and Transcriptome Analysis. J. Bacteriol. 190: 3161-3168 [Abstract] [Full Text]  
  • Walter, J., Schwab, C., Loach, D. M., Ganzle, M. G., Tannock, G. W. (2008). Glucosyltransferase A (GtfA) and inulosucrase (Inu) of Lactobacillus reuteri TMW1.106 contribute to cell aggregation, in vitro biofilm formation, and colonization of the mouse gastrointestinal tract. Microbiology 154: 72-80 [Abstract] [Full Text]  
  • Santos, F., Vera, J. L., van der Heijden, R., Valdez, G., de Vos, W. M., Sesma, F., Hugenholtz, J. (2008). The complete coenzyme B12 biosynthesis gene cluster of Lactobacillus reuteri CRL1098. Microbiology 154: 81-93 [Abstract] [Full Text]  
  • Denou, E., Berger, B., Barretto, C., Panoff, J.-M., Arigoni, F., Brussow, H. (2007). Gene Expression of Commensal Lactobacillus johnsonii Strain NCC533 during In Vitro Growth and in the Murine Gut. J. Bacteriol. 189: 8109-8119 [Abstract] [Full Text]  
  • Cai, H., Rodriguez, B. T., Zhang, W., Broadbent, J. R., Steele, J. L. (2007). Genotypic and phenotypic characterization of Lactobacillus casei strains isolated from different ecological niches suggests frequent recombination and niche specificity. Microbiology 153: 2655-2665 [Abstract] [Full Text]  
  • Hufner, E., Markieton, T., Chaillou, S., Crutz-Le Coq, A.-M., Zagorec, M., Hertel, C. (2007). Identification of Lactobacillus sakei Genes Induced during Meat Fermentation and Their Role in Survival and Growth. Appl. Environ. Microbiol. 73: 2522-2531 [Abstract] [Full Text]  
  • Marco, M. L., Bongers, R. S., de Vos, W. M., Kleerebezem, M. (2007). Spatial and Temporal Expression of Lactobacillus plantarum Genes in the Gastrointestinal Tracts of Mice. Appl. Environ. Microbiol. 73: 124-132 [Abstract] [Full Text]  
  • Tannock, G. W., Ghazally, S., Walter, J., Loach, D., Brooks, H., Cook, G., Surette, M., Simmers, C., Bremer, P., Dal Bello, F., Hertel, C. (2005). Ecological Behavior of Lactobacillus reuteri 100-23 Is Affected by Mutation of the luxS Gene. Appl. Environ. Microbiol. 71: 8419-8425 [Abstract] [Full Text]  
  • Dal Bello, F., Walter, J., Roos, S., Jonsson, H., Hertel, C. (2005). Inducible Gene Expression in Lactobacillus reuteri LTH5531 during Type II Sourdough Fermentation. Appl. Environ. Microbiol. 71: 5873-5878 [Abstract] [Full Text]  
  • Rediers, H., Rainey, P. B., Vanderleyden, J., De Mot, R. (2005). Unraveling the Secret Lives of Bacteria: Use of In Vivo Expression Technology and Differential Fluorescence Induction Promoter Traps as Tools for Exploring Niche-Specific Gene Expression. Microbiol. Mol. Biol. Rev. 69: 217-261 [Abstract] [Full Text]  
  • Oozeer, R., Furet, J. P., Goupil-Feuillerat, N., Anba, J., Mengaud, J., Corthier, G. (2005). Differential Activities of Four Lactobacillus casei Promoters during Bacterial Transit through the Gastrointestinal Tracts of Human-Microbiota-Associated Mice. Appl. Environ. Microbiol. 71: 1356-1363 [Abstract] [Full Text]  
  • Walter, J., Chagnaud, P., Tannock, G. W., Loach, D. M., Dal Bello, F., Jenkinson, H. F., Hammes, W. P., Hertel, C. (2005). A High-Molecular-Mass Surface Protein (Lsp) and Methionine Sulfoxide Reductase B (MsrB) Contribute to the Ecological Performance of Lactobacillus reuteri in the Murine Gut. Appl. Environ. Microbiol. 71: 979-986 [Abstract] [Full Text]  
  • Desmond, C., Fitzgerald, G. F., Stanton, C., Ross, R. P. (2004). Improved Stress Tolerance of GroESL-Overproducing Lactococcus lactis and Probiotic Lactobacillus paracasei NFBC 338. Appl. Environ. Microbiol. 70: 5929-5936 [Abstract] [Full Text]  
  • Bron, P. A., Grangette, C., Mercenier, A., de Vos, W. M., Kleerebezem, M. (2004). Identification of Lactobacillus plantarum Genes That Are Induced in the Gastrointestinal Tract of Mice. J. Bacteriol. 186: 5721-5729 [Abstract] [Full Text]  
  • Tannock, G. W. (2004). A Special Fondness for Lactobacilli. Appl. Environ. Microbiol. 70: 3189-3194 [Full Text]  
  • Zoetendal, E. G., Collier, C. T., Koike, S., Mackie, R. I., Gaskins, H. R. (2004). Molecular Ecological Analysis of the Gastrointestinal Microbiota: A Review. J. Nutr. 134: 465-472 [Abstract] [Full Text]  
  • Xu, J., Gordon, J. I. (2003). Inaugural Article: Honor thy symbionts. Proc. Natl. Acad. Sci. USA 100: 10452-10459 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»