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Applied and Environmental Microbiology, April 2003, p. 2073-2079, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2073-2079.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Exopolysaccharide and Kestose Production by Lactobacillus sanfranciscensis LTH2590

Maher Korakli, Melanie Pavlovic, Michael G. Gänzle,* and Rudi F. Vogel

Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Freising, Germany

Received 16 August 2002/ Accepted 19 January 2003

The effect was investigated of sucrose concentration on sucrose metabolism and on the formation of exopolysaccharide (EPS) by Lactobacillus sanfranciscensis LTH2590 in pH-controlled fermentations with sucrose concentrations ranging from 20 to 160 g liter-1. The EPS production increased and the relative sucrose hydrolysis activity decreased by increasing the sucrose concentration in the medium. The carbon recovery decreased from 95% at a sucrose concentration of 30 g liter-1 to 58% at a sucrose concentration of 160 g liter-1 because of the production of an unknown metabolite by L. sanfranciscensis. This metabolite was characterized as a fructo-oligosaccharide. The oligosaccharide produced by L. sanfranciscensis was purified and characterized as a trisaccharide with a glucose/fructose ratio of 1:2. The comparison of the retention time of this oligosaccharide and that of pure oligosaccharide standards using two different chromatography methods revealed that the oligosaccharide produced by L. sanfranciscensis LTH2590 is 1-kestose. Kestose production increased concomitantly with the initial sucrose concentration in the medium.

* Corresponding author. Mailing address: TU-München, Lehrstuhl für Technische Mikrobiologie, Weihenstephaner Steig 16, 85350 Freising, Germany. Phone: 49 0 8161 71 3204. Fax: 49 0 8161 71 3327. E-mail: michael.gaenzle{at}wzw.tum.de.

Applied and Environmental Microbiology, April 2003, p. 2073-2079, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2073-2079.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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