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Applied and Environmental Microbiology, April 2003, p. 2201-2208, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2201-2208.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Interstrain Inhibition in the Sweet Potato Pathogen Streptomyces ipomoeae: Purification and Characterization of a Highly Specific Bacteriocin and Cloning of Its Structural Gene

Xiujun Zhang,1,{dagger} Christopher A. Clark,2 and Gregg S. Pettis1*

Department of Biological Sciences, Louisiana State University,1 Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Louisiana Agricultural Experiment Station, Baton Rouge, Louisiana 708032

Received 20 September 2002/ Accepted 7 January 2003

Strains of the sweet potato soil rot pathogen Streptomyces ipomoeae had previously been divided into three groups based on their ability to inhibit one another during pairwise cocultivation. While group I strains are not antagonistic to members of the other groups, group II and group III strains produce separate substances that are inhibitory to strains outside their respective cognate groups. Here, we purified the group III inhibitory substance from the culture supernatant of a representative strain and found that it consists of a single 10-kDa cationic protein which is bacteriolytic for S. ipomoeae group I and II strains but which showed no inhibitory function against other streptomycetes or other bacterial genera tested. The structural gene for the inhibitor was cloned from a chromosomal library of the producing strain, and while the gene sequence revealed that the inhibitor is initially made in a larger precursor form, the deduced mature protein showed no significant homology to other known proteins. Our results demonstrate that S. ipomoeae group III inhibitory activity is manifested in the form of a highly specific, potentially novel bacteriocin, which we have designated ipomicin.

* Corresponding author. Mailing address: Department of Biological Sciences, Louisiana State University, 202 Life Sciences Building, Baton Rouge, LA 70803-1715. Phone: (225) 578-2798. Fax: (225) 578-2597. E-mail: gpettis{at}lsu.edu.

{dagger} Present address: Department of Chemistry, Rice University, Houston, TX 77005.

Applied and Environmental Microbiology, April 2003, p. 2201-2208, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2201-2208.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wang, J., Schully, K. L., Pettis, G. S. (2009). Growth-Regulated Expression of a Bacteriocin, Produced by the Sweet Potato Pathogen Streptomyces ipomoeae, That Exhibits Interstrain Inhibition. Appl. Environ. Microbiol. 75: 1236-1242 [Abstract] [Full Text]  
  • Holtsmark, I., Mantzilas, D., Eijsink, V. G. H., Brurberg, M. B. (2006). Purification, Characterization, and Gene Sequence of Michiganin A, an Actagardine-Like Lantibiotic Produced by the Tomato Pathogen Clavibacter michiganensis subsp. michiganensis. Appl. Environ. Microbiol. 72: 5814-5821 [Abstract] [Full Text]  


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