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Applied and Environmental Microbiology, April 2003, p. 2245-2252, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2245-2252.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Killing of Bacillus Spores by Aqueous Dissolved Oxygen, Ascorbic Acid, and Copper Ions

J. B. Cross,1 R. P. Currier,1* D. J. Torraco,1 L. A. Vanderberg,1 G. L. Wagner,2 and P. D. Gladen2

Chemistry Division,1 Biosciences Division, Los Alamos National Laboratory, Los Alamos, New Mexico 875452

Received 16 August 2002/ Accepted 26 December 2002

An approach to decontamination of biological endospores is discussed. Specifically, the performance of an aqueous modified Fenton reagent is examined. A modified Fenton reagent formulation of cupric chloride, ascorbic acid, and sodium chloride is shown to be an effective sporicide under aerobic conditions. The traditional Fenton reaction involves the conversion of hydrogen peroxide to hydroxyl radical by aqueous ionic catalysts such as the transition metal ions. Our modified Fenton reaction involves the conversion of aqueous dissolved oxygen to hydrogen peroxide by an ionic catalyst (Cu2+) and then subsequent conversion to hydroxyl radicals. Results are given for the modified Fenton reagent deactivating spores of Bacillus globigii. A biocidal mechanism is proposed that is consistent with our experimental results and independently derived information found in the literature. This mechanism requires diffusion of relatively benign species into the interior of the spore, where dissolved O2 is then converted through a series of reactions which ultimately produce hydroxyl radicals that perform the killing action.

* Corresponding author. Mailing address: Chemistry Division, Los Alamos National Laboratory, Los Alamos, NM 87545. Phone: (505) 665-3601. Fax: (505) 667-0440. E-mail: currier{at}lanl.gov.

Applied and Environmental Microbiology, April 2003, p. 2245-2252, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2245-2252.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shapiro, M. P., Setlow, B., Setlow, P. (2004). Killing of Bacillus subtilis Spores by a Modified Fenton Reagent Containing CuCl2 and Ascorbic Acid. Appl. Environ. Microbiol. 70: 2535-2539 [Abstract] [Full Text]  


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