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Applied and Environmental Microbiology, April 2003, p. 2356-2360, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2356-2360.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Multiplex Reverse Transcription-PCR for Simultaneous Detection of Beet Necrotic Yellow Vein Virus, Beet Soilborne Virus, and Beet Virus Q and Their Vector Polymyxa betae KESKIN on Sugar Beet

Alexandre Meunier,1 Jean-François Schmit,1 Arnaud Stas,1 Nazli Kutluk,2 and Claude Bragard1*

Unité de Phytopathologie-UCL-AGRO-BAPA, B-1348 Louvain-la-Neuve, Belgium,1 Plant Protection Department, Agricultural Faculty, Gaziosmanpasa University, 60240 Tasliciftlik, Tokat, Turkey2

Received 12 September 2002/ Accepted 2 January 2003

Three soilborne viruses transmitted by Polymyxa betae KESKIN in sugar beet have been described: Beet necrotic yellow vein virus (BNYVV), the agent of rhizomania, Beet soilborne virus (BSBV), and Beet virus Q (BVQ). A multiplex reverse transcription-PCR technique was developed to simultaneously detect BNYVV, BSBV, and BVQ, together with their vector, P. betae. The detection threshold of the test was up to 128 times greater than that of an enzyme-linked immunosorbent assay. Systematic association of BNYVV with one or two different pomoviruses was observed. BVQ was detected in samples from Belgium, Bulgaria, France, Germany, Hungary, Italy, Sweden, and The Netherlands but not in samples from Turkey.

* Corresponding author. Mailing address: Unité de Phytopathologie-UCL-AGRO-BAPA, Place Croix du Sud, Bte 3, B-1348 Louvain-la-Neuve, Belgium. Phone: 32 10 47 37 47. Fax: 32 10 47 86 97. E-mail: meunier{at}fymy.ucl.ac.be.

Applied and Environmental Microbiology, April 2003, p. 2356-2360, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2356-2360.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Crutzen, F., Mehrvar, M., Gilmer, D., Bragard, C. (2009). A full-length infectious clone of beet soil-borne virus indicates the dispensability of the RNA-2 for virus survival in planta and symptom expression on Chenopodium quinoa leaves. J. Gen. Virol. 90: 3051-3056 [Abstract] [Full Text]  
  • Crutzen, F., Kreit, M., Bragard, C. (2009). The beet virus Q coat protein readthrough domain is longer than previously reported, with two transmembrane domains. J. Gen. Virol. 90: 754-758 [Abstract] [Full Text]  
  • Schirmer, A., Link, D., Cognat, V., Moury, B., Beuve, M., Meunier, A., Bragard, C., Gilmer, D., Lemaire, O. (2005). Phylogenetic analysis of isolates of Beet necrotic yellow vein virus collected worldwide. J. Gen. Virol. 86: 2897-2911 [Abstract] [Full Text]  


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