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Applied and Environmental Microbiology, April 2003, p. 2365-2371, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2365-2371.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Heat Shock Response by the Hyperthermophilic Archaeon Pyrococcus furiosus

Keith R. Shockley, Donald E. Ward,{dagger} Swapnil R. Chhabra,{ddagger} Shannon B. Conners, Clemente I. Montero, and Robert M. Kelly*

Department of Chemical Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905

Received 12 November 2002/ Accepted 15 January 2003

Collective transcriptional analysis of heat shock response in the hyperthermophilic archaeon Pyrococcus furiosus was examined by using a targeted cDNA microarray in conjunction with Northern analyses. Differential gene expression suggests that P. furiosus relies on a cooperative strategy of rescue (thermosome [Hsp60], small heat shock protein [Hsp20], and two VAT-related chaperones), proteolysis (proteasome), and stabilization (compatible solute formation) to cope with polypeptide processing during thermal stress.

* Corresponding author. Mailing address: Department of Chemical Engineering, North Carolina State University, Raleigh, NC 27695-7905. Phone: (919) 515-6396. Fax: (919) 515-3465. E-mail: rmkelly{at}eos.ncsu.edu.

{dagger} Present address: Genencor International, Inc., Palo Alto, CA 94304-1013.

{ddagger} Present address: Biosystems Research Department, Sandia National Laboratories, Livermore, CA 94551-0969.

Applied and Environmental Microbiology, April 2003, p. 2365-2371, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2365-2371.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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