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Applied and Environmental Microbiology, April 2003, p. 2386-2388, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2386-2388.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Surface-Associated and Secreted MopE Protein of Methylococcus capsulatus (Bath) Responds to Changes in the Concentration of Copper in the Growth Medium

Odd A. Karlsen,1 Frode S. Berven,1 Graham P. Stafford,2,{dagger} Øivind Larsen,1 J. Colin Murrell,2 Harald B. Jensen,1 and Anne Fjellbirkeland1*

Department of Molecular Biology, University of Bergen, 5020 Bergen, Norway,1 Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom2

Received 3 September 2002/ Accepted 15 January 2003

Expression of surface-associated and secreted protein MopE of the methanotrophic bacterium Methylococcus capsulatus (Bath) in response to the concentration of copper ions in the growth medium was investigated. The level of protein associated with the cells and secreted to the medium changed when the copper concentration in the medium varied and was highest in cells exposed to copper stress.

* Corresponding author. Mailing address: Department of Molecular Biology, University of Bergen, Thormøhlensgt. 55, 5020 Bergen, Norway. Phone: 47 55584500. Fax: 47 55589683. E-mail: Anne.Fjellbirkeland{at}mbi.uib.no.

{dagger} Present address: Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom.

Applied and Environmental Microbiology, April 2003, p. 2386-2388, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2386-2388.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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