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Applied and Environmental Microbiology, April 2003, p. 2399-2404, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2399-2404.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Release of Extracellular Transformable Plasmid DNA from Escherichia coli Cocultivated with Algae

Kazuaki Matsui,1* Nobuyoshi Ishii,2 and Zen'ichiro Kawabata1

Center for Ecological Research, Kyoto University, Otsu 520-2113,1 Environmental and Toxicological Science Research Group, National Institute of Radiological Sciences, Inage-ku, Chiba-shi 263-8555, Japan2

Received 12 August 2002/ Accepted 13 January 2003

We studied the effects of cocultivation with either Euglena gracilis (Euglenophyta), Microcystis aeruginosa (Cyanophyta), Chlamydomonas neglecta (Chlorophyta), or Carteria inversa (Chlorophyta) on the production of extracellular plasmid DNA by Escherichia coli LE392(pKZ105). Dot blot hybridization analysis showed a significant release of plasmid DNA by cocultivation with all the algae tested. Further analysis by electrotransformation confirmed the release of transformable plasmid DNA by cocultivation with either E. gracilis, M. aeruginosa, or C. inversa. These results suggest algal involvement in bacterial horizontal gene transfer by stimulating the release of transformable DNA into aquatic environments.

* Corresponding author. Mailing address: Center for Ecological Research, Kyoto University, Kamitanakami Hirano-cho 509-3, Otsu 520-2113, Japan. Phone: 81-077-549-8234. Fax: 81-077-549-8201. E-mail: k-matsui{at}ecology.kyoto-u.ac.jp.

Applied and Environmental Microbiology, April 2003, p. 2399-2404, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.2399-2404.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ishii, N., Uchida, S. (2006). Removal of Technetium from Solution by Algal Flagellate Euglena gracilis. J. Environ. Qual. 35: 2017-2020 [Abstract] [Full Text]  
  • Steinberger, R. E., Holden, P. A. (2005). Extracellular DNA in Single- and Multiple-Species Unsaturated Biofilms. Appl. Environ. Microbiol. 71: 5404-5410 [Abstract] [Full Text]  


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