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Applied and Environmental Microbiology, May 2003, p. 2580-2586, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2580-2586.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Growth Characteristics and Intraspecies Host Specificity of a Large Virus Infecting the Dinoflagellate Heterocapsa circularisquama

Keizo Nagasaki,1* Yuji Tomaru,2 Kenji Tarutani,1 Noriaki Katanozaka,3 Satoshi Yamanaka,3 Hiroshi Tanabe,3 and Mineo Yamaguchi1

National Research Institute of Fisheries and Environment of Inland Sea,1 and Japan Society for the Promotion of Science, Ohno, Saeki, Hiroshima 739-0452,2 SDS Biotech K.K., Tsukuba, Ibaraki 300-2646, Japan3

Received 5 August 2002/ Accepted 12 February 2003

The growth characteristics and intraspecies host specificity of Heterocapsa circularisquama virus (HcV), a large icosahedral virus specifically infecting the bivalve-killing dinoflagellate H. circularisquama, were examined. Exponentially growing host cells were more sensitive to HcV than those in the stationary phase, and host cells were more susceptible to HcV infection in the culture when a higher percent of the culture was replaced with fresh medium each day, suggesting an intimate relationship between virus sensitivity and the physiological condition of the host cells. HcV was infective over a wide range of temperatures, 15 to 30°C, and the latent period and burst size were estimated at 40 to 56 h and 1,800 to 2,440 infective particles, respectively. Transmission electron microscopy revealed that capsid formation began within 16 h postinfection, and mature virus particles appeared within 24 h postinfection at 20°C. Compared to Heterosigma akashiwo virus, HcV was more widely infectious to H. circularisquama strains that had been independently isolated in the western part of Japan, and only 5.3% of the host-virus combinations (53 host and 10 viral strains) showed resistance to viral infection. The present results are helpful in understanding the ecology of algal host-virus systems in nature.

* Corresponding author. Mailing address: 2-17-5 Maruishi, Ohno, Saeki, Hiroshima 739-0452, Japan. Phone: 81 829 55 0666. Fax: 81 829 54 1216. E-mail: nagasaki{at}affrc.go.jp.

Applied and Environmental Microbiology, May 2003, p. 2580-2586, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2580-2586.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nagasaki, K., Tomaru, Y., Katanozaka, N., Shirai, Y., Nishida, K., Itakura, S., Yamaguchi, M. (2004). Isolation and Characterization of a Novel Single-Stranded RNA Virus Infecting the Bloom-Forming Diatom Rhizosolenia setigera. Appl. Environ. Microbiol. 70: 704-711 [Abstract] [Full Text]  


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