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Applied and Environmental Microbiology, May 2003, p. 2603-2607, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2603-2607.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A Novel NAD-Dependent Dehydrogenase, Highly Specific for 1,5-Anhydro-D-Glucitol, from Trichoderma longibrachiatum Strain 11-3

Nobuyuki Yoshida,* Etsuko Uchida, Tohoru Katsuragi, and Yoshiki Tani

Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan

Received 31 October 2002/ Accepted 28 January 2003

A novel NAD-dependent dehydrogenase highly specific for 1,5-anhydro-D-glucitol (1,5-AG) was found in the cell extract of an imperfect fungus, Trichoderma longibrachiatum strain 11-3. This fungus used 1,5-AG as a sole carbon source for growth and transformed 1,5-AG into glucose. 1,5-AG dehydrogenase (AGH) was purified to homogeneity, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular mass of the purified enzyme was estimated to be 36 and 141 kDa by SDS-PAGE and by gel filtration, respectively, suggesting that the enzyme was homotetrameric. The enzyme was highly specific for 1,5-AG and did not exhibit activity with any sugar or sugar alcohol tested in this study other than 1,5-AG. A linear relationship between the initial rate of the enzyme reaction and the concentration of 1,5-AG at the physiological level was observed. The presence of glucose in abundance did not interfere with the relationship. The optimum temperature for the enzyme reaction was 50°C, and the enzyme was stable at temperatures up to 70°C. These results suggested that AGH is a novel enzyme and is useful for specifically diagnosing diabetes mellitus.

* Corresponding author. Mailing address: School of Biosciences, NAIST, Ikoma 630-0192, Japan. Phone: 81-743-72-5423. Fax: 81-743-72-5429. E-mail: yoshidan{at}bs.aist-nara.ac.jp.

Applied and Environmental Microbiology, May 2003, p. 2603-2607, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2603-2607.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kuhn, A., Yu, S., Giffhorn, F. (2006). Catabolism of 1,5-Anhydro-D-Fructose in Sinorhizobium morelense S-30.7.5: Discovery, Characterization, and Overexpression of a New 1,5-Anhydro-D-Fructose Reductase and Its Application in Sugar Analysis and Rare Sugar Synthesis. Appl. Environ. Microbiol. 72: 1248-1257 [Abstract] [Full Text]  


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