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Applied and Environmental Microbiology, May 2003, p. 2875-2878, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2875-2878.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Protocol for Rapid Fluorescence In Situ Hybridization of Bacteria in Cryosections of Microarthropods

Torsten Thimm and Christoph C. Tebbe*

Institut für Agrarökologie, Bundesforschunganstalt für Landwirtschaft (FAL), D-38116 Braunschweig, Germany

Received 13 September 2002/ Accepted 23 January 2003

A protocol was developed to detect bacteria inhabiting microarthropods by means of small-subunit rRNA-targeted fluorescence in situ hybridization and microscopy. The protocol is based on cryosections of whole specimens. In contrast to more commonly applied paraffin-embedding techniques, the protocol is quicker and reduces the number of manipulations which might damage the microscopic material. The method allowed the study of the bacterial colonization of Folsomia candida (Collembola) and the detection of bacteria in both the gut and tissue.

* Corresponding author. Mailing address: Institut für Agrarökologie, Bundesforschunganstalt für Landwirtschaft (FAL), Bundesallee 50, 38116 Braunschweig, Germany. Phone: (49) 531 596 2553. Fax: (49) 531 596 2599. E-mail: christoph.tebbe{at}fal.de.

Applied and Environmental Microbiology, May 2003, p. 2875-2878, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2875-2878.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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