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Applied and Environmental Microbiology, May 2003, p. 2936-2941, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2936-2941.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of an Emergent and Atypical Pseudomonas viridiflava Lineage Causing Bacteriosis in Plants of Agronomic Importance in a Spanish Region

Ana J. González,1 M. Rosario Rodicio,2 and M. Carmen Mendoza2*

Servicio Regional de Investigación y Desarrollo Agroalimentario, Principado de Asturias, Villaviciosa,1 Departamento de Biología Funcional, Area Microbiología, Universidad de Oviedo, Oviedo, Spain2

Received 6 December 2002/ Accepted 24 February 2003

Pseudomonas strains with an atypical LOPAT profile (where LOPAT is a series of determinative tests: L, levan production; O, oxidase production; P, pectinolitic activity; A, arginine dihydrolase production; and T, tobacco hypersensibility) can be regarded as emergent pathogens in the Principality of Asturias (Spain), where they have been causing, since 1999, severe damage in at least three taxonomically unrelated orchard plants of agronomic importance: common bean (Phaseolus vulgaris), kiwifruit (Actinidia deliciosa), and lettuce (Lactuca sativa). These strains are mainly differentiated by production of yellowish mucoid material in hypersucrose medium, used for the levan test, and by a variable pectinolytic activity on different potato varieties. The atypical organisms were identified as Pseudomonas viridiflava based on their 16S rRNA sequences. Among them a certain intraspecies genetic heterogeneity was detected by randomly amplified polymorphic DNA (RAPD) typing. To differentiate between isolates of P. viridiflava and Pseudomonas syringae pathovars, a 16S ribosomal DNA restriction fragment length polymorphism method employing the restriction endonucleases SacI and HinfI was developed. This could be used as a means of reliable species determination after the usual phenotypical characterization, which includes the LOPAT tests.

* Corresponding author. Mailing address: Area de Microbiología. Facultad de Medicina, C/Julián Clavería 6, 33006 Oviedo, Spain. Phone: 34 985103560. Fax: 34 985103148. E-mail: cmendoza{at}correo.uniovi.es.

Applied and Environmental Microbiology, May 2003, p. 2936-2941, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2936-2941.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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