This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hölscher, T.
Right arrow Articles by Adrian, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hölscher, T.
Right arrow Articles by Adrian, L.
Agricola
Right arrow Articles by Hölscher, T.
Right arrow Articles by Adrian, L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2003, p. 2999-3001, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2999-3001.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Reductive Dehalogenation of Chlorobenzene Congeners in Cell Extracts of Dehalococcoides sp. Strain CBDB1

Tina Hölscher,* Helmut Görisch, and Lorenz Adrian

Fachgebiet Technische Biochemie, Institut für Biotechnologie, Technische Universität Berlin, D-13353 Berlin, Germany

Received 27 September 2002/ Accepted 13 February 2003

Enzymatic reductive dehalogenation of tri-, tetra-, penta-, and hexachlorobenzenes was demonstrated in cell extracts with low protein concentration (0.5 to 1 µg of protein/ml) derived from the chlorobenzene-respiring anaerobe Dehalococcoides sp. strain CBDB1. 1,2,3-trichlorobenzene dehalogenase activity was associated with the membrane fraction. Light-reversible inhibition by alkyl iodides indicated the presence of a corrinoid cofactor.

* Corresponding author. Mailing address: FG Technische Biochemie, Sekr. GG1, TU Berlin, Seestr. 13, D-13353 Berlin, Germany. Phone: 49 30 31427509. Fax: 49 30 31427581. E-mail: tina.hoelscher{at}tu-berlin.de.

Applied and Environmental Microbiology, May 2003, p. 2999-3001, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2999-3001.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wagner, A., Adrian, L., Kleinsteuber, S., Andreesen, J. R., Lechner, U. (2009). Transcription Analysis of Genes Encoding Homologues of Reductive Dehalogenases in "Dehalococcoides" sp. Strain CBDB1 by Using Terminal Restriction Fragment Length Polymorphism and Quantitative PCR. Appl. Environ. Microbiol. 75: 1876-1884 [Abstract] [Full Text]  
  • Rowe, A. R., Lazar, B. J., Morris, R. M., Richardson, R. E. (2008). Characterization of the Community Structure of a Dechlorinating Mixed Culture and Comparisons of Gene Expression in Planktonic and Biofloc-Associated "Dehalococcoides" and Methanospirillum Species. Appl. Environ. Microbiol. 74: 6709-6719 [Abstract] [Full Text]  
  • Behrens, S., Azizian, M. F., McMurdie, P. J., Sabalowsky, A., Dolan, M. E., Semprini, L., Spormann, A. M. (2008). Monitoring Abundance and Expression of "Dehalococcoides" Species Chloroethene-Reductive Dehalogenases in a Tetrachloroethene-Dechlorinating Flow Column. Appl. Environ. Microbiol. 74: 5695-5703 [Abstract] [Full Text]  
  • Adrian, L., Rahnenfuhrer, J., Gobom, J., Holscher, T. (2007). Identification of a Chlorobenzene Reductive Dehalogenase in Dehalococcoides sp. Strain CBDB1. Appl. Environ. Microbiol. 73: 7717-7724 [Abstract] [Full Text]  
  • Fung, J. M., Morris, R. M., Adrian, L., Zinder, S. H. (2007). Expression of Reductive Dehalogenase Genes in Dehalococcoides ethenogenes Strain 195 Growing on Tetrachloroethene, Trichloroethene, or 2,3-Dichlorophenol. Appl. Environ. Microbiol. 73: 4439-4445 [Abstract] [Full Text]  
  • Grostern, A., Edwards, E. A. (2006). A 1,1,1-Trichloroethane-Degrading Anaerobic Mixed Microbial Culture Enhances Biotransformation of Mixtures of Chlorinated Ethenes and Ethanes. Appl. Environ. Microbiol. 72: 7849-7856 [Abstract] [Full Text]  
  • Ritalahti, K. M., Amos, B. K., Sung, Y., Wu, Q., Koenigsberg, S. S., Loffler, F. E. (2006). Quantitative PCR Targeting 16S rRNA and Reductive Dehalogenase Genes Simultaneously Monitors Multiple Dehalococcoides Strains. Appl. Environ. Microbiol. 72: 2765-2774 [Abstract] [Full Text]  
  • Regeard, C., Maillard, J., Dufraigne, C., Deschavanne, P., Holliger, C. (2005). Indications for Acquisition of Reductive Dehalogenase Genes through Horizontal Gene Transfer by Dehalococcoides ethenogenes Strain 195. Appl. Environ. Microbiol. 71: 2955-2961 [Abstract] [Full Text]  
  • Nijenhuis, I., Zinder, S. H. (2005). Characterization of Hydrogenase and Reductive Dehalogenase Activities of Dehalococcoides ethenogenes Strain 195. Appl. Environ. Microbiol. 71: 1664-1667 [Abstract] [Full Text]  
  • Holscher, T., Krajmalnik-Brown, R., Ritalahti, K. M., von Wintzingerode, F., Gorisch, H., Loffler, F. E., Adrian, L. (2004). Multiple Nonidentical Reductive-Dehalogenase-Homologous Genes Are Common in Dehalococcoides. Appl. Environ. Microbiol. 70: 5290-5297 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»