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Applied and Environmental Microbiology, May 2003, p. 3011-3014, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.3011-3014.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Production of Native-Type Streptoverticillium mobaraense Transglutaminase in Corynebacterium glutamicum

Masayo Date, Kei-ichi Yokoyama, Yukiko Umezawa, Hiroshi Matsui, and Yoshimi Kikuchi*

Institute of Life Sciences, Ajinomoto Co., Inc., Kawasaki 210-8681, Japan

Received 11 November 2002/ Accepted 28 February 2003

We previously observed secretion of active-form transglutaminase in Corynebacterium glutamicum by coexpressing the subtilisin-like protease SAM-P45 from Streptomyces albogriseolus to process the prodomain. However, the N-terminal amino acid sequence of the transglutaminase differed from that of the native Streptoverticillium mobaraense enzyme. In the present work we have used site-directed mutagenesis to generate an optimal SAM-P45 cleavage site in the C-terminal region of the prodomain. As a result, native-type transglutaminase was secreted.

* Corresponding author. Mailing address: Institute of Life Sciences, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210-8681, Japan. Phone: (81)-44-244-7123. Fax: (81)-44-222-0129. E-mail: yoshimi_kikuchi{at}ajinomoto.com.

Applied and Environmental Microbiology, May 2003, p. 3011-3014, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.3011-3014.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Watanabe, K., Tsuchida, Y., Okibe, N., Teramoto, H., Suzuki, N., Inui, M., Yukawa, H. (2009). Scanning the Corynebacterium glutamicum R genome for high-efficiency secretion signal sequences. Microbiology 155: 741-750 [Abstract] [Full Text]  
  • Kikuchi, Y., Itaya, H., Date, M., Matsui, K., Wu, L.-F. (2009). TatABC Overexpression Improves Corynebacterium glutamicum Tat-Dependent Protein Secretion. Appl. Environ. Microbiol. 75: 603-607 [Abstract] [Full Text]  
  • Kikuchi, Y., Date, M., Itaya, H., Matsui, K., Wu, L.-F. (2006). Functional Analysis of the Twin-Arginine Translocation Pathway in Corynebacterium glutamicum ATCC 13869. Appl. Environ. Microbiol. 72: 7183-7192 [Abstract] [Full Text]  


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