This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kovács, A. T.
Right arrow Articles by Kovács, K. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kovács, A. T.
Right arrow Articles by Kovács, K. L.
Agricola
Right arrow Articles by Kovács, A. T.
Right arrow Articles by Kovács, K. L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2003, p. 3093-3102, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3093-3102.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Genes Involved in the Biosynthesis of Photosynthetic Pigments in the Purple Sulfur Photosynthetic Bacterium Thiocapsa roseopersicina

Ákos T. Kovács, Gábor Rákhely, and Kornél L. Kovács*

Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, and Department of Biotechnology, University of Szeged, H-6726 Szeged, Hungary

Received 19 December 2002/ Accepted 26 February 2003

A pigment mutant strain of the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina BBS was isolated by plasposon mutagenesis. Nineteen open reading frame, most of which are thought to be genes involved in the biosynthesis of carotenoids, bacteriochlorophyll, and the photosynthetic reaction center, were identified surrounding the plasposon in a 22-kb-long chromosomal locus. The general arrangement of the photosynthetic genes was similar to that in other purple photosynthetic bacteria; however, the locations of a few genes occurring in this region were unusual. Most of the gene products showed the highest similarity to the corresponding proteins in Rubrivivax gelatinosus. The plasposon was inserted into the crtD gene, likely inactivating crtC as well, and the carotenoid composition of the mutant strain corresponded to the aborted spirilloxanthin pathway. Homologous and heterologous complementation experiments indicated a conserved function of CrtC and CrtD in the purple photosynthetic bacteria. The crtDC and crtE genes were shown to be regulated by oxygen, and a role of CrtJ in aerobic repression was suggested.

* Corresponding author. Mailing address: Department of Biotechnology, University of Szeged, Temesvári krt. 62, H-6726 Szeged, Hungary. Phone: 36 62 544 351. Fax: 36 62 544 352. E-mail: kornel{at}nucleus.szbk.u-szeged.hu.

Applied and Environmental Microbiology, June 2003, p. 3093-3102, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3093-3102.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Klassen, J. L. (2009). Pathway Evolution by Horizontal Transfer and Positive Selection Is Accommodated by Relaxed Negative Selection upon Upstream Pathway Genes in Purple Bacterial Carotenoid Biosynthesis. J. Bacteriol. 191: 7500-7508 [Abstract] [Full Text]  
  • Maroti, G., Tong, Y., Yooseph, S., Baden-Tillson, H., Smith, H. O., Kovacs, K. L., Frazier, M., Venter, J. C., Xu, Q. (2009). Discovery of [NiFe] Hydrogenase Genes in Metagenomic DNA: Cloning and Heterologous Expression in Thiocapsa roseopersicina. Appl. Environ. Microbiol. 75: 5821-5830 [Abstract] [Full Text]  
  • Sun, Z., Shen, S., Wang, C., Wang, H., Hu, Y., Jiao, J., Ma, T., Tian, B., Hua, Y. (2009). A novel carotenoid 1,2-hydratase (CruF) from two species of the non-photosynthetic bacterium Deinococcus. Microbiology 155: 2775-2783 [Abstract] [Full Text]  
  • Tian, B., Sun, Z., Xu, Z., Shen, S., Wang, H., Hua, Y. (2008). Carotenoid 3',4'-desaturase is involved in carotenoid biosynthesis in the radioresistant bacterium Deinococcus radiodurans. Microbiology 154: 3697-3706 [Abstract] [Full Text]  
  • Kovacs, A. T., Rakhely, G., Browning, D. F., Fulop, A., Maroti, G., Busby, S. J. W., Kovacs, K. L. (2005). An FNR-Type Regulator Controls the Anaerobic Expression of Hyn Hydrogenase in Thiocapsa roseopersicina. J. Bacteriol. 187: 2618-2627 [Abstract] [Full Text]  
  • Aklujkar, M., Prince, R. C., Beatty, J. T. (2005). The PuhB Protein of Rhodobacter capsulatus Functions in Photosynthetic Reaction Center Assembly with a Secondary Effect on Light-Harvesting Complex 1. J. Bacteriol. 187: 1334-1343 [Abstract] [Full Text]  
  • Fodor, B. D., Kovacs, A. T., Csaki, R., Hunyadi-Gulyas, E., Klement, E., Maroti, G., Meszaros, L. S., Medzihradszky, K. F., Rakhely, G., Kovacs, K. L. (2004). Modular Broad-Host-Range Expression Vectors for Single-Protein and Protein Complex Purification. Appl. Environ. Microbiol. 70: 712-721 [Abstract] [Full Text]  
  • Rakhely, G., Kovacs, A. T., Maroti, G., Fodor, B. D., Csanadi, G., Latinovics, D., Kovacs, K. L. (2004). Cyanobacterial-Type, Heteropentameric, NAD+-Reducing NiFe Hydrogenase in the Purple Sulfur Photosynthetic Bacterium Thiocapsa roseopersicina. Appl. Environ. Microbiol. 70: 722-728 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»