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Applied and Environmental Microbiology, June 2003, p. 3103-3109, Vol. 69, No. 6
0099-2240/03/$08.00+0 DOI: 10.1128/AEM.69.6.3103-3109.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Department of Medical Microbiology, Dermatology and Infection, University of Lund, S-223 62 Lund, Sweden
Received 9 September 2002/ Accepted 7 March 2003
An integrated procedure is presented whereby gas chromatography-ion trap mass spectrometry is used to determine chemical markers of gram-negative bacterial lipopolysaccharide (3-hydroxy fatty acids with 10 to 18 carbon atoms), gram-positive bacteria (branched-chain fatty acids with 15 and 17 carbon atoms), bacterial peptidoglycan (muramic acid), and fungal biomass (ergosterol) in samples of settled house dust. A hydrolysate of 13C-labeled cyanobacterial cells is used as an internal standard for the first three markers. These analyses require two dust samples, one for 3-OH fatty acids, branched-chain fatty acids, and muramic acid and another for ergosterol. The method may be used to characterize microbial communities in environmental samples.
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