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Applied and Environmental Microbiology, June 2003, p. 3103-3109, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3103-3109.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Characterization of the Microbial Community in Indoor Environments: a Chemical-Analytical Approach

Aleksandra Sebastian and Lennart Larsson*

Department of Medical Microbiology, Dermatology and Infection, University of Lund, S-223 62 Lund, Sweden

Received 9 September 2002/ Accepted 7 March 2003

An integrated procedure is presented whereby gas chromatography-ion trap mass spectrometry is used to determine chemical markers of gram-negative bacterial lipopolysaccharide (3-hydroxy fatty acids with 10 to 18 carbon atoms), gram-positive bacteria (branched-chain fatty acids with 15 and 17 carbon atoms), bacterial peptidoglycan (muramic acid), and fungal biomass (ergosterol) in samples of settled house dust. A hydrolysate of 13C-labeled cyanobacterial cells is used as an internal standard for the first three markers. These analyses require two dust samples, one for 3-OH fatty acids, branched-chain fatty acids, and muramic acid and another for ergosterol. The method may be used to characterize microbial communities in environmental samples.

* Corresponding author. Mailing address: Department of Medical Microbiology, Dermatology and Infection, University of Lund, Sölvegatan 23, S-223 62 Lund, Sweden. Phone: 46 46 177298. Fax: 46 46 189117. E-mail: Lennart.Larsson{at}mmb.lu.se.

Applied and Environmental Microbiology, June 2003, p. 3103-3109, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3103-3109.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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