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Applied and Environmental Microbiology, June 2003, p. 3137-3143, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3137-3143.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of Listeria monocytogenes Genes Involved in Salt and Alkaline-pH Tolerance

Rozenn Gardan,1* Pascale Cossart,2 The European Listeria Genome Consortium,{dagger} and Jean Labadie1

Station de Recherches sur la Viande, Institut National de la Recherche Agronomique, Theix, 63122 Saint-Genès Champanelle,1 Unité des Interactions Bactéries-Cellules, Institut Pasteur, 75724 Paris Cedex 15, France2

Received 10 December 2002/ Accepted 18 March 2003

The capacity of Listeria monocytogenes to tolerate salt and alkaline stresses is of particular importance, as this pathogen is often exposed to such environments during food processing and food preservation. We screened a library of Tn917-lacZ insertional mutants in order to identify genes involved in salt and/or alkaline tolerance. We isolated six mutants sensitive to salt stress and 12 mutants sensitive to salt and alkaline stresses. The position of the insertion of the transposon was located in 15 of these mutants. In six mutants the transposon was inserted in intergenic regions, and in nine mutants it was inserted in genes. Most of the genes have unknown functions, but sequence comparisons indicated that they encode putative transporters.

* Corresponding author. Present address: Unité de Biochimie et Structure des Protéines, Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas Cedex, France. Phone: 33-1-34-65-27-70. Fax: 33-1-34-65-21-63. E-mail: rgardan{at}jouy.inra.fr.

{dagger} Members of The European Listeria Genome Consortium are listed in Acknowledgments.

Applied and Environmental Microbiology, June 2003, p. 3137-3143, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3137-3143.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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