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Applied and Environmental Microbiology, June 2003, p. 3152-3157, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3152-3157.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Aerobic Denitrifying Bacteria That Produce Low Levels of Nitrous Oxide

Naoki Takaya,1 Maria Antonina B. Catalan-Sakairi,1 Yasushi Sakaguchi,1 Isao Kato,1 Zhemin Zhou,1 and Hirofumi Shoun2*

Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572,1 Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-Ku, Tokyo 113-8657, Japan2

Received 25 November 2002/ Accepted 17 March 2003

Most denitrifiers produce nitrous oxide (N2O) instead of dinitrogen (N2) under aerobic conditions. We isolated and characterized novel aerobic denitrifiers that produce low levels of N2O under aerobic conditions. We monitored the denitrification activities of two of the isolates, strains TR2 and K50, in batch and continuous cultures. Both strains reduced nitrate (NO3-) to N2 at rates of 0.9 and 0.03 µmol min-1 unit of optical density at 540 nm-1 at dissolved oxygen (O2) (DO) concentrations of 39 and 38 µmol liter-1, respectively. At the same DO level, the typical denitrifier Pseudomonas stutzeri and the previously described aerobic denitrifier Paracoccus denitrificans did not produce N2 but evolved more than 10-fold more N2O than strains TR2 and K50 evolved. The isolates denitrified NO3- with concomitant consumption of O2. These results indicated that strains TR2 and K50 are aerobic denitrifiers. These two isolates were taxonomically placed in the ß subclass of the class Proteobacteria and were identified as P. stutzeri TR2 and Pseudomonas sp. strain K50. These strains should be useful for future investigations of the mechanisms of denitrifying bacteria that regulate N2O emission, the single-stage process for nitrogen removal, and microbial N2O emission into the ecosystem.

* Corresponding author. Mailing address: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-Ku, Tokyo 113-8657, Japan. Phone and fax: 81-35841-5148. E-mail: ahshoun{at}mail.ecc.u-tokyo.ac.jp.

Applied and Environmental Microbiology, June 2003, p. 3152-3157, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3152-3157.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hernandez-Ramirez, G., Brouder, S. M., Smith, D. R., Van Scoyoc, G. E., Michalski, G. (2009). Nitrous Oxide Production in an Eastern Corn Belt Soil: Sources and Redox Range. Soil Sci. 73: 1182-1191 [Abstract] [Full Text]  
  • Toyofuku, M., Nomura, N., Kuno, E., Tashiro, Y., Nakajima, T., Uchiyama, H. (2008). Influence of the Pseudomonas Quinolone Signal on Denitrification in Pseudomonas aeruginosa. J. Bacteriol. 190: 7947-7956 [Abstract] [Full Text]  


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