This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matsuda, M.
Right arrow Articles by Moore, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matsuda, M.
Right arrow Articles by Moore, J. E.
Agricola
Right arrow Articles by Matsuda, M.
Right arrow Articles by Moore, J. E.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2003, p. 3308-3310, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3308-3310.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Characterization of Urease-Positive Thermophilic Campylobacter Subspecies by Multilocus Enzyme Electrophoresis Typing

Motoo Matsuda,1 Aki Kaneko,1 Timothy Stanley,2 B. Cherie Millar,2 M. Miyajima,1 Philip G. Murphy,2 and John E. Moore2*

Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Sagamihara 229, Japan,1 Northern Ireland Public Health Laboratory, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland, United Kingdom2

Received 9 September 2002/ Accepted 19 February 2003

Thirty-one urease-positive thermophilic Campylobacter (UPTC) isolates, including three reference strains (NCTC12892, NCTC12895 and NCTC12896), and three Campylobacter lari isolates, which were isolated from several countries and sources, were compared genotypically by using multilocus enzyme electrophoresis (MLEE). We examined allelic variation around seven enzyme loci, including the adenylate kinase, alkaline phosphatase, catalase, fumarase, malic enzyme, malate dehydrogenase, and L-phenylalanyl-L-leucine peptidase loci. MLEE typing revealed the presence of 23 different electrophoretic types (ETs) among the 31 UPTC isolates, and 14 isolates shared six electrophoretic profiles. Three different ETs were identified for the three C. lari isolates examined, and no ETs were shared by UPTC and C. lari isolates. Quantitative analyses were subsequently performed by using allelic variation data, and the results demonstrated that the mean genetic diversity was 0.655. In conclusion, MLEE demonstrated that the UPTC isolates examined are genetically hypervariable and form a cluster separate from the C. lari cluster.

* Corresponding author. Mailing address: Northern Ireland Public Health Laboratory, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland, United Kingdom. Phone: 44(28) 9026 3554. Fax: 44(28) 2589 2887. E-mail: jemoore{at}niphl.dnet.co.uk.

Applied and Environmental Microbiology, June 2003, p. 3308-3310, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3308-3310.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Debruyne, L., On, S. L. W., De Brandt, E., Vandamme, P. (2009). Novel Campylobacter lari-like bacteria from humans and molluscs: description of Campylobacter peloridis sp. nov., Campylobacter lari subsp. concheus subsp. nov. and Campylobacter lari subsp. lari subsp. nov.. Int. J. Syst. Evol. Microbiol. 59: 1126-1132 [Abstract] [Full Text]  
  • Klena, J. D., Parker, C. T., Knibb, K., Ibbitt, J. C., Devane, P. M. L., Horn, S. T., Miller, W. G., Konkel, M. E. (2004). Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a Multiplex PCR Developed from the Nucleotide Sequence of the Lipid A Gene lpxA. J. Clin. Microbiol. 42: 5549-5557 [Abstract] [Full Text]  
  • Matsuda, M., Moore, J. E. (2004). Urease-Positive Thermophilic Campylobacter Species. Appl. Environ. Microbiol. 70: 4415-4418 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»