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Applied and Environmental Microbiology, June 2003, p. 3333-3343, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3333-3343.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification and Manipulation of Soil Properties To Improve the Biological Control Performance of Phenazine-Producing Pseudomonas fluorescens

Bonnie H. Ownley,^1* Brion K. Duffy,² and David M. Weller³

Department of Entomology and Plant Pathology, The University of Tennessee, Knoxville, Tennessee 37996,¹ Swiss Federal Research Station for Fruit, Wine, and Vegetable Production, Wädenswil, Switzerland CH-8820,² Cereal Root Disease and Biological Control Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164³

Received 30 August 2002/ Accepted 10 March 2003

Pseudomonas fluorescens 2-79RN₁₀ protects wheat against take-all disease caused by Gaeumannomyces graminis var. tritici; however, the level of protection in the field varies from site to site. Identification of soil factors that exert the greatest influence on disease suppression is essential to improving biocontrol. In order to assess the relative importance of 28 soil properties on take-all suppression, seeds were treated with strain 2-79RN₁₀ (which produces phenazine-1-carboxylate [PCA⁺]) or a series of mutants with PCA⁺ and PCA^- phenotypes. Bacterized seeds were planted in 10 soils, representative of the wheat-growing region in the Pacific Northwest. Sixteen soil properties were correlated with disease suppression. Biocontrol activity of PCA⁺ strains was positively correlated with ammonium-nitrogen, percent sand, soil pH, sodium (extractable and soluble), sulfate-sulfur, and zinc. In contrast, biocontrol was negatively correlated with cation-exchange capacity (CEC), exchangeable acidity, iron, manganese, percent clay, percent organic matter (OM), percent silt, total carbon, and total nitrogen. Principal component factor analysis of the 16 soil properties identified a three-component solution that accounted for 87 percent of the variance in disease rating (biocontrol). A model was identified with step-wise regression analysis (R² = 0.96; Cp statistic = 6.17) that included six key soil properties: ammonium-nitrogen, CEC, iron, percent silt, soil pH, and zinc. As predicted by our regression model, the biocontrol activity of 2-79RN₁₀ was improved by amending a soil low in Zn with 50 µg of zinc-EDTA/g of soil. We then investigated the negative correlation of OM with disease suppression and found that addition of OM (as wheat straw) at rates typical of high-OM soils significantly reduced biocontrol activity of 2-79RN₁₀.

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* Corresponding author. Mailing address: Department of Entomology and Plant Pathology, 205 Ellington Plant Sciences Bldg., The University of Tennessee, 2431 Center Dr., Knoxville, TN 37996. Phone: (865) 974-0219. Fax: (865) 974-4744. E-mail: bownley{at}utk.edu.

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Applied and Environmental Microbiology, June 2003, p. 3333-3343, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3333-3343.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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