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Applied and Environmental Microbiology, June 2003, p. 3462-3468, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3462-3468.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Glucose Metabolism in Lactococcus lactis MG1363 under Different Aeration Conditions: Requirement of Acetate To Sustain Growth under Microaerobic Conditions

Mikkel Nordkvist, Niels Bang Siemsen Jensen,{dagger} and John Villadsen*

Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby, Denmark

Received 10 September 2002/ Accepted 18 March 2003

Lactococcus lactis subsp. lactis MG1363 was grown in batch cultures on a defined medium with glucose as the energy source under different aeration conditions, namely, anaerobic conditions, aerobic conditions, and microaerobic conditions with a dissolved oxygen tension of 5% (when saturation with air was used as the reference). The maximum specific growth rate was high (0.78 to 0.91 h-1) under all aeration conditions but decreased with increasing aeration, and more than 90% of the glucose was converted to lactate. However, a shift in by-product formation was observed. Increasing aeration resulted in acetate, CO2, and acetoin replacing formate and ethanol as end products. Under microaerobic conditions, growth came to a gradual halt, although more than 60% of the glucose was still left. A decline in growth was not observed during microaerobic cultivation when acetate was added to the medium. We hypothesize that the decline in growth was due to a lack of acetyl coenzyme A (acetyl-CoA) needed for fatty acid synthesis since acetyl-CoA can be synthesized from acetate by means of acetate kinase and phosphotransacetylase activities.

* Corresponding author. Mailing address: Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, Building 223, DK-2800 Lyngby, Denmark. Phone: (45) 45252668. Fax: (45) 45884148. E-mail: jv{at}biocentrum.dtu.dk.

{dagger} Present address: Chr. Hansen A/S, DK-2970 Hørsholm, Denmark.

Applied and Environmental Microbiology, June 2003, p. 3462-3468, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3462-3468.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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